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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium.
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Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium.

机译：表没食子儿茶素没食子酸酯在各种细胞培养基中的不同细胞毒性和致胶裂作用，由于其在培养基中的氧化速率不同。

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摘要

Positive genotoxicity results are often observed using mammalian cells in culture with agents that are not in vivo genotoxins. We here illustrate one possible explanation: interaction of test chemicals with the cell-culture media used. We find that the toxicity and clastogenicity of epigallocatechin gallate (EGCG) to Chinese Hamster ovary (CHO) cells is affected by the culture medium used and appears largely or entirely due to variable rates of formation of hydrogen peroxide (H(2)O(2)) by chemical reactions of EGCG with the culture media. Catalase decreased EGCG toxicity substantially. Of seven different types of commonly used media evaluated, F-10 and F-12 nutrient mixtures were the least prone to produce this artefact. Although it generated H(2)O(2) in the culture media, ascorbate was not toxic to CHO cells because the H(2)O(2) levels achieved were insufficient to kill these cells. Thus, the culture medium, the cell type and the presence or absence of catalase (e.g. its variable amounts in S9 fractions) must be taken into account in in vitro genotoxicity testing.
机译：使用哺乳动物细胞与非体内基因毒素的试剂一起培养时，通常会观察到阳性的遗传毒性结果。我们在这里举例说明一种可能的解释：测试化学品与所用细胞培养基的相互作用。我们发现表没食子儿茶素没食子酸酯（EGCG）对中国仓鼠卵巢（CHO）细胞的毒性和致死性受所用培养基的影响，并且在很大程度上或完全由于过氧化氢（H（2）O（2 ））EGCG与培养基的化学反应。过氧化氢酶大大降低了EGCG的毒性。在评估的七种不同类型的常用培养基中，F-10和F-12营养混合物最不易产生这种假象。尽管它在培养基中产生H（2）O（2），但抗坏血酸对CHO细胞无毒，因为所达到的H（2）O（2）水平不足以杀死这些细胞。因此，在体外遗传毒性测试中，必须考虑培养基，细胞类型和过氧化氢酶的存在与否（例如，过氧化氢酶在S9组分中的含量可变）。

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