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首页> 外文期刊>Mutation Research. Reviews in Mutation Research >Learning to 'think like a geneticist' with the de Serres ad-3 forward-mutation test in Neurospora crassa
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Learning to 'think like a geneticist' with the de Serres ad-3 forward-mutation test in Neurospora crassa

机译：通过神经孢菌中的de Serres ad-3正向突变测试学习“像遗传学家一样思考”

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When I joined the laboratory of Frederick J. de Serres in the Biology Division of the Oak Ridge National Laboratory as a Postdoctoral Fellow in 1960, he was developing the adenine-3 (ad-3) for-ward-mutation test in Neurospora crassa. This test utilizes a direct method based on the accumulation of purple pigment by ad-3 mutants, rather than their requirement for adenine [1]. Control or treated conidia are incubated in 12-1 Florence flasks containing 10 1 of minimal medium supplemented with sorbose, sucrose, and a low concentration of adenine. After 7 days of incubation with aeration in the dark at 30 deg C, the purple (ad-3 mutant) colonies contrast with the background of white (wild-type) colonies. I had proposed to use this intriguing new test to determine the mutagenicity of certain base analogs, especially 2-aminopurine, in N. crassa. As a graduate student (1956-1960), I had read with great interest (and probably without much understanding) the papers of Benzer and Freese on the various aspects of the mutagenicity of base analogs, such as 2-aminopurine, in the rII region of bacteriophage T4 [2-5]. Later, I came to recognize these papers as classics among the early papers on proposed molecular mechanisms of mutation, and I have long included them in my genetics courses.
机译：1960年，我以博士后身份加入了橡树岭国家实验室生物学部门的Frederick J. de Serres实验室时，他正在开发Neurospora crassa中的腺嘌呤3（ad-3）正向突变测试。该测试利用一种直接方法，该方法基于ad-3突变体积累的紫色色素，而不是其对腺嘌呤的要求[1]。将对照或处理过的分生孢子在12-1弗洛伦斯烧瓶中孵育，其中装有10 1补充有山梨糖，蔗糖和低浓度腺嘌呤的基本培养基。在黑暗中于30摄氏度通气孵育7天后，紫色（ad-3突变体）菌落与白色（野生型）菌落的背景形成对比。我曾提议使用这种吸引人的新测试来确定某些基本类似物，尤其是2-氨基嘌呤在克氏猪笼草中的致突变性。作为一名研究生（1956-1960年），我非常感兴趣（并且可能没有太多了解）阅读了Benzer和Freese的论文，涉及rII区碱基类似物（如2-氨基嘌呤）的致突变性的各个方面。 T4噬菌体[2-5]。后来，我逐渐认识到这些论文是有关拟议的突变分子机制的早期论文中的经典著作，并且长期以来我一直将它们纳入我的遗传学课程。

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