...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Cytokinesis-blocked micronucleus cytome and comet assays in peripheral blood lymphocytes of workers exposed to lead considering folate and vitamin B12 status.
【24h】

Cytokinesis-blocked micronucleus cytome and comet assays in peripheral blood lymphocytes of workers exposed to lead considering folate and vitamin B12 status.

机译：考虑到叶酸和维生素B12的状态，接触铅的工人外周血淋巴细胞的胞质分裂阻滞性微核细胞计数和彗星测定。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In human biomonitoring, factors such as age, gender, smoking habit and alcohol consumption are usually considered and identified as agents that exert an impact on genotoxicity biomarkers. However, as a rule, factors like micronutrient status are not considered in biomonitoring studies. This paper reports on genotoxic damage in Pb-exposed workers using data obtained from the cytokinesis-block micronucleus (CBMN) cytome assay and alkaline comet assay with silver staining, considering folate and vitamin B12 nutritional status. Analysis of the results showed that the Pb-exposed group presented a 24-fold higher Pb content in the blood compared to controls. The Pb-exposed workers presented significantly greater micronuclei (MNi) counts (Z=7.9583; p<0.0001) and DNA damage, assessed by the comet assay (DF: Z=7.7056; p<0.0001 and DI: Z=7.4749; p<0.0001), but no correlation with Pb blood concentrations were detected. These two groups did not differ significantly concerning folate and vitamin B12 levels. Regarding folate, it is possible that this similarity is associated with folic acid flour-enrichment, introduced in Brazil in 2004 to prevent neural tube defects. The mean folate level obtained in the Pb-exposed group was 6.18ng/ml. The subgroup of individuals with serum folate levels equal to or higher than the mean presented significantly higher MNi (Z=2.3776; p=0.017) and nucleoplasmic bridge (NPB) frequencies (Z=1.9850; p=0.047) in peripheral blood lymphocytes. A significant positive correlation was observed between the age of Pb-exposed workers and MNi frequencies (Gamma=0.3328; p<0.001), NPBs (Gamma=0.1832; p<0.042) and DNA damage assessed by the comet assay (DF: Gamma=0.1764; p=0.035 and DI: Gamma=0.11852; p=0.028). These findings suggest that high folate levels alone do not guarantee protection against genotoxic damage. Moreover, folic acid supplementation should be studied using more efficient approaches to determine safe amounts and potential deleterious effects.
机译：在人类生物监测中，通常考虑并确定诸如年龄，性别，吸烟习惯和饮酒等因素，这些因素会对遗传毒性生物标志物产生影响。但是，通常在生物监测研究中不考虑微量营养素状态等因素。本文利用叶酸和维生素B12的营养状况，通过细胞分裂阻滞微核（CBMN）细胞计数法和碱性彗星测定法（银染）获得的数据，对暴露于铅的工人进行了遗传毒性损害研究。结果分析表明，与对照组相比，接触Pb的人群血液中Pb含量高24倍。暴露于铅的工人表现出显着更高的微核（MNi）计数（Z = 7.9583; p <0.0001）和DNA损伤（通过彗星试验评估）（DF：Z = 7.7056; p <0.0001和DI：Z = 7.4749; p < 0.0001），但未检测到与Pb血药浓度的相关性。两组在叶酸和维生素B12水平方面无显着差异。关于叶酸，这种相似性可能与2004年在巴西引入的防止神经管缺陷的叶酸粉浓缩有关。铅暴露组的平均叶酸水平为6.18ng / ml。血清叶酸水平等于或高于平均值的亚组在外周血淋巴细胞中表现出显着更高的MNi（Z = 2.3776; p = 0.017）和核质桥（NPB）频率（Z = 1.9850; p = 0.047）。铅暴露工人的年龄与MNi频率（Gamma = 0.3328; p <0.001），NPBs（Gamma = 0.1832; p <0.042）和彗星试验评估的DNA损伤之间具有显着正相关（DF：Gamma = 0.1764; p = 0.035和DI：Gamma = 0.11852; p = 0.028）。这些发现表明，仅叶酸水平过高并不能保证免受遗传毒性损害。此外，应使用更有效的方法研究叶酸补充剂，以确定安全量和潜在的有害影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号