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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Micronucleus formation, DNA damage and repair in premenopausal women chronically exposed to high level of indoor air pollution from biomass fuel use in rural India.
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Micronucleus formation, DNA damage and repair in premenopausal women chronically exposed to high level of indoor air pollution from biomass fuel use in rural India.

机译：在印度农村地区，长期暴露于因使用生物质燃料而产生的高水平室内空气污染的绝经前妇女中，微核的形成，DNA的损伤和修复。

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Genotoxicity of indoor air pollution from biomass fuel use has been examined in 132 biomass users (median age 34 years) and 85 age-matched control women from eastern India who used the cleaner fuel liquefied petroleum gas (LPG) to cook. Micronucleus (MN) frequency was evaluated in buccal (BEC) and airway epithelial cells (AEC); DNA damage was examined by comet assay in peripheral blood lymphocytes (PBL); and expressions of gamma-H2AX, Mre11 and Ku70 proteins were localized in AEC and PBL by immunocytochemistry. Reactive oxygen species (ROS) generation in leukocytes was measured by flow cytometry, and the levels of superoxide dismutase (SOD) and total antioxidant status (TAS) in blood were measured by spectrophotometry. Real-time aerosol monitor was used to measure particulate pollutants in indoor air. Compared with controls, biomass users had increased frequencies of micronucleated cells in BEC (3.5 vs. 1.7, p<0.001) and AEC (4.54 vs. 1.86, p<0.001), and greater comet tail % DNA (18.6 vs. 11.7%, p<0.01), tail length (45.5 vs. 31.4mum, p<0.01) and olive tail moment (4.0 vs. 1.4, p<0.01) in PBL. Moreover, biomass users had more gamma-H2AX-positive nuclei in PBL (49.5 vs. 8.5%, p<0.01) and AEC (11.3 vs. 2.9%, p<0.01) along with higher expression of DNA repair proteins Mre11 and Ku70 in these cells, suggesting stimulation of DNA repair mechanism. Biomass users showed rise in ROS generation and depletion of SOD and TAS. Biomass-using households had 2-4 times more particulate matter with diameter less than 10 and 2.5mum in indoor air, and MN frequency and comet tail % DNA were positively associated with these pollutants after controlling potential confounders. Thus, chronic exposure to biomass smoke causes chromosomal and DNA damage and upregulation of DNA repair mechanism.
机译：已调查了来自印度东部的132位使用生物质燃料的室内空气污染的遗传毒性（中位年龄34岁）和来自印度东部的85位年龄匹配的对照妇女，她们使用更清洁的燃料液化石油气（LPG）进行烹饪。在颊（BEC）和气道上皮细胞（AEC）中评估微核（MN）频率;通过彗星试验检测外周血淋巴细胞（PBL）中的DNA损伤;免疫细胞化学法检测γ-H2AX，Mre11和Ku70蛋白在AEC和PBL中的表达。通过流式细胞术测量白细胞中活性氧（ROS）的生成，并通过分光光度法测量血液中超氧化物歧化酶（SOD）和总抗氧化剂状态（TAS）的水平。实时气溶胶监测仪用于测量室内空气中的颗粒污染物。与对照组相比，生物量使用者在BEC（3.5 vs. 1.7，p <0.001）和AEC（4.54 vs. 1.86，p <0.001）中微核细胞的频率增加，并且彗尾DNA百分比更高（18.6 vs. 11.7％， P <0.01），PBL中的尾巴长度（45.5对31.4毫米，p <0.01）和橄榄尾矩（4.0对1.4，p <0.01）。此外，生物量使用者在PBL（49.5 vs. 8.5％，p <0.01）和AEC（11.3 vs. 2.9％，p <0.01）中具有更多的γ-H2AX阳性核，并且DNA修复蛋白Mre11和Ku70的表达更高。这些细胞，提示刺激DNA修复机制。生物量使用者显示ROS的产生以及SOD和TAS的消耗增加。在控制潜在的混杂因素之后，使用生物质的家庭的室内空气中直径小于10和2.5mum的颗粒物多于2-4倍，而MN频率和彗尾DNA百分比与这些污染物呈正相关。因此，长期暴露于生物质烟雾会导致染色体和DNA损伤以及DNA修复机制的上调。

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