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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Mitomycin C, 5-fluoruracil, colchicine and etoposide tested in the in vitro mammalian cell micronucleus test (MNvit) in the human lymphoblastoid cell line TK6 at Novartis in support of OECD draft Test Guideline 487.
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Mitomycin C, 5-fluoruracil, colchicine and etoposide tested in the in vitro mammalian cell micronucleus test (MNvit) in the human lymphoblastoid cell line TK6 at Novartis in support of OECD draft Test Guideline 487.

机译：丝裂霉素C，5-氟尿嘧啶，秋水仙碱和依托泊苷在诺华公司的人淋巴母细胞系TK6的体外哺乳动物细胞微核试验（MNvit）中进行了试验，以支持OECD测试指南487。

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摘要

The following reference genotoxic agents were tested in the in vitro micronucleus test, at Novartis, Basel, Switzerland. Mitomycin C, 5-fluoruracil, colchicine and etoposide were tested in the human lymphoblastoid cell line TK6, with and without cytokinesis block (in the presence of cytochalasin B). This was done in support of the toxicity measures recommended in the draft OECD Test Guideline on In Vitro Mammalian Cell Micronucleus Test (MNvit) and was part of an international collaborative work. As toxicity measures, detecting cytostasis and cell death, relative cell counts (RCC), relative increase in cell counts (RICC), and relative population doubling (RPD) were used for treatments in the absence of cytokinesis block, and replication index (RI) or cytokinesis-blocked proliferation in the presence of cytokinesis block. All four reference agents were positive in the assay with and without cytokinesis block at concentrations giving approximately 50% toxicity or less as assessed by all of the toxicity measures used. Accordingly, the results of this work support the use of relative population doubling and relative increase in cell counts, as well as relative cell counts, as appropriate measures of toxicity for the non-cytokinesis-blocked in vitro micronucleus assay.
机译：在瑞士巴塞尔的诺华公司的体外微核试验中，对以下参考遗传毒性剂进行了试验。丝裂霉素C，5-氟尿嘧啶，秋水仙碱和依托泊苷在具有和不具有胞质分裂阻滞作用（在细胞松弛素B存在下）的人淋巴母细胞系TK6中进行了测试。这样做是为了支持OECD体外哺乳动物细胞微核试验（MNvit）试验指南草案中建议的毒性措施，并且是国际合作工作的一部分。在检测无细胞分裂阻滞和复制指数（RI）的治疗方法中，使用毒性检测方法来检测细胞停滞和细胞死亡，相对细胞计数（RCC），相对细胞计数（RICC）和相对群体倍增（RPD）。存在胞质分裂阻滞时，细胞分裂或胞质分裂阻滞增生。通过使用的所有毒性测量方法评估，在有或没有胞质分裂阻滞的情况下，所有四种参考试剂均为阳性，浓度约为50％或更低。因此，这项工作的结果支持使用相对群体倍增和细胞计数以及相对细胞计数的相对增加，作为对非胞质分裂阻滞的体外微核试验毒性的适当测量。

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