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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (comet) assay. 2. Relationship between DNA migration and alkaline condition.
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Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (comet) assay. 2. Relationship between DNA migration and alkaline condition.

机译：使用单细胞凝胶电泳（彗星）测定法检测化学诱变剂诱导的DNA损伤。 2. DNA迁移与碱性条件之间的关系。

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摘要

The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assayto detect the genotoxic effects of chemicals.
机译：碱性条件是碱性单细胞凝胶电泳（SCG）分析检测化学物质遗传毒性作用的重要因素。为了了解DNA迁移与碱性条件之间的关系，在两种不同的碱性条件（pH 12.1和12.6）下，通过碱性SCG分析评估了13种具有不同作用模式的化学诱变剂的作用。处理1小时后立即取样CHO细胞。 X射线模拟诱变剂BLM在pH 12.1和12.6时增加了DNA迁移，在两个pH值下结果均相同。六个烷基化诱变剂MNU，ENU，MNNG，ENNG，MMS和EMS和一个碱基加合物诱导剂4-NQO仅在pH 12.6时诱导剂量依赖性反应。两种DNA交联剂，MMC和DDP，以及AMD均呈阴性结果。但是，MMC和DDP降低了BLM的阳性反应，表明可以检测到DNA交联。这些结果表明，碱性条件是碱性SCG分析检测化学物质遗传毒性作用的重要因素。

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