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首页> 外文期刊>Molecular cell >Inter-RNA interaction of phage phi29 pRNA to form a hexameric complex for viral DNA transportation.
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Inter-RNA interaction of phage phi29 pRNA to form a hexameric complex for viral DNA transportation.

机译：噬菌体phi29 pRNA的RNA间相互作用形成了病毒DNA运输的六聚体复合物。

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摘要

Ds-DNA viruses package their DNA into a preformed protein shell (procapsid) during maturation. Bacteriophage phi29 requires an RNA (pRNA) to package its genomic DNA into the procapsid. We report here that the pRNA upper and lower loops are involved in RNA/RNA interactions. Mutation in only one loop results in inactive pRNAs. However, mixing of two, three and six inactive mutant pRNAs restores DNA packaging activity as long as an interlocking hexameric ring can be predicted to form by base pairing of the mutated loops in separate RNA molecules. The stoichiometry of pRNA for the packaging of one viral DNA genome is six. Homogeneous pRNA purified from a single band in denaturing gels showed six bands when rerun in native gels. These results suggest that six pRNAs form a hexameric ring by the intermolecular interaction of two RNA loops to serve as part of the DNA transportation machinery.
机译：Ds-DNA病毒在成熟过程中将其DNA包装到预先形成的蛋白质外壳（前壳体）中。噬菌体phi29需要RNA（pRNA）才能将其基因组DNA包装到前壳体中。我们在这里报告说，pRNA的上部和下部环参与RNA / RNA相互作用。仅一个环中的突变会导致pRNA失活。但是，混合两个，三个和六个无活性的突变pRNA可以恢复DNA的包装活性，只要可以通过单独的RNA分子中突变环的碱基配对预测形成一个互锁的六聚环。包装一个病毒DNA基因组的pRNA的化学计量为6。从变性凝胶中的单个条带纯化的均质pRNA在天然凝胶中重新运行时显示6条带。这些结果表明，六个pRNA通过两个RNA环的分子间相互作用形成一个六聚环，以作为DNA转运机制的一部分。

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