Arginine/Serine-Rich Domains of SR Proteins Can Function as Activators of Pre-mRNA Splicing

Brenton R. Graveley; Tom Maniatis

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Arginine/Serine-Rich Domains of SR Proteins Can Function as Activators of Pre-mRNA Splicing

机译：SR蛋白的精氨酸/富含丝氨酸的域可以充当pre-mRNA剪接的激活剂。

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Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS domains in splicing enhancer function. Hybrid proteins containing RS domains fused to the MS2 RNA binding protein were tested in vitro the RNA substrates bearing an MS2 recognition sequence. These hybrid proteins activated splicing in nuclear extracts, but not in S100 extracts lacking SR proteins. However, intact recombinant SR proteins could complement the activity of the hybrid proteins in S100 extracts. These data demonstrate that RS domains function as splicing activators and suggest that the general and enhancer-dependent functions of SR proteins can Be uncoupled.

机译：富含丝氨酸/精氨酸（SR）的剪接因子包含RNA结合结构域和蛋白质-蛋白质相互作用所需的富含精氨酸/丝氨酸（RS）的结构域。除了在基本剪接反应中的作用外，SR蛋白还作为剪接增强复合物的成分。在这里，我们研究了RS域在剪接增强子功能中的作用。在体外测试了带有与MS2 RNA结合蛋白融合的RS结构域的杂合蛋白，该底物带有MS2识别序列。这些杂合蛋白在核提取物中激活了剪接，但在缺少SR蛋白的S100提取物中未激活剪接。但是，完整的重组SR蛋白可以补充S100提取物中杂合蛋白的活性。这些数据表明，RS结构域起着剪接激活剂的作用，表明SR蛋白的一般功能和增强子依赖性功能可以解偶联。

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《Molecular cell》 |1998年第5期|共7页
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Brenton R. Graveley; Tom Maniatis;
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中图分类分子生物学;细胞生物学;
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1. Arginine/Serine-Rich Domains of SR Proteins Can Function as Activators of Pre-mRNA Splicing [J] . Brenton R. Graveley, Tom Maniatis Molecular cell . 1998,第5期

机译：SR蛋白的精氨酸/富含丝氨酸的域可以充当pre-mRNA剪接的激活剂。
2. Identification of a pre-mRNA splicing factor, arginine/serine-rich 3 (Sfrs3), and its co-expression with fibronectin in fetal and postnatal rabbit airway mucosal and skin wounds [J] . Li-Korotky HS, Hebda PA, Kelly LA, Biochimica et biophysica acta. Molecular basis of disease: BBA . 2006,第1期

机译：鉴定mRNA前剪接因子精氨酸/富含丝氨酸的3（Sfrs3）及其与纤连蛋白在胎儿和产后兔气道粘膜和皮肤伤口中的共表达
3. Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins [J] . Heim A, Grimm C, Muller U, Nucleic Acids Research . 2014,第12期

机译：含有Jumonji域的蛋白6（Jmjd6）调节剪接并与SR和SR样蛋白的富含精氨酸丝氨酸（RS）的域特异性相互作用
4. Identification of methylation and phosphorylation in arginine/serine-rich domains by electron transfer dissociation mass spectrometry [C] . Isaac Bishof, Duc Duong, Ian Diner, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：电子转移解离质谱法识别鉴定精氨酸/丝氨酸富域中的甲基化和磷酸化
5. Protein Arginine Methylation Modulates pre-mRNA Splicing in Saccharomyces cerevisiae. [D] . Jackson, Christopher. 2016

机译：蛋白精氨酸甲基化调节酿酒酵母中的前mRNA剪接。
6. Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine–serine-rich (RS) domains of SR- and SR-like proteins [O] . Astrid Heim, Christina Grimm, Udo Müller, 2014

机译：包含蛋白质6（Jmjd6）的Jumonji域调节剪接并与SR和SR样蛋白的富含精氨酸-丝氨酸（RS）的域特异性相互作用
7. Identification of a pre-mRNA splicing factor, arginine/serine-rich 3 (Sfrs3), and its co-expression with fibronectin in fetal and postnatal rabbit airway mucosal and skin wounds [O] . Li-Korotky Ha-Sheng, Hebda Patricia A., Kelly Lori A., 2006

机译：鉴定mRNA前剪接因子精氨酸/富含丝氨酸的3（Sfrs3）及其与纤连蛋白在胎儿和产后兔气道粘膜和皮肤伤口中的共表达

Arginine/Serine-Rich Domains of SR Proteins Can Function as Activators of Pre-mRNA Splicing

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