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首页> 外文期刊>Molecular cell >Nucleosome Stability Distinguishes Two Different Promoter Types at All Protein-Coding Genes in Yeast
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Nucleosome Stability Distinguishes Two Different Promoter Types at All Protein-Coding Genes in Yeast

机译：核糖体稳定性区分酵母中所有蛋白质编码基因的两种不同的启动子类型

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摘要

Previous studies indicate that eukaryotic promoters display a stereotypical chromatin landscape characterized by a well-positioned +1 nucleosome near the transcription start site and an upstream 1 nucleosome that together demarcate a nucleosome-free (or -depleted) region. Here we present evidence that there are two distinct types of promoters distinguished by the resistance of the -1 nucleosome to micrococcal nuclease digestion. These different architectures are characterized by two sequence motifs that are broadly deployed at one set of promoters where a nuclease-sensitive ("fragile'') nucleosome forms, but concentrated in a narrower, nucleosome-free region at all other promoters. The RSC nucleosome remodeler acts through the motifs to establish stable +1 and -1 nucleosome positions, while binding of a small set of general regulatory (pioneer) factors at fragile nucleosome promoters plays a key role in their destabilization. We propose that the fragile nucleosome promoter architecture is adapted for regulation of highly expressed, growth-related genes.
机译：先前的研究表明，真核启动子显示出定型染色质景观，其特征是靠近转录起始位点的+1核小体定位良好，而上游1核小体共同界定了无核小体（或耗尽）区域。在这里，我们提供的证据表明，有两种不同类型的启动子，它们以-1核小体对微球菌核酸酶消化的抗性来区分。这些不同的体系结构的特征是两个序列基序，它们广泛分布在一组启动子上，在这些启动子上形成了核酸酶敏感（“易碎”）核小体，但在所有其他启动子上集中在一个较窄的无核小体的区域。重塑剂通过这些基序起作用，以建立稳定的+1和-1核小体位置，而在脆弱的核小体启动子上结合少量的一般调节因子（先驱因子）则在其不稳定中起关键作用。适用于调节高度表达的生长相关基因。

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