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首页> 外文期刊>Molecular cell >ResT, a Telomere Resolvase Encoded by the Lyme Disease Spirochete
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ResT, a Telomere Resolvase Encoded by the Lyme Disease Spirochete

机译：ResT，一种由莱姆病螺旋体编码的端粒解离酶

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摘要

The genus Borrelia includes the causative agents of Lyme disease and relapsing fever. An unusual feature of these bacteria is a segmented genome consisting mostly of a number of linear DNA molecules with covalently closed hairpin ends or telomeres. In this study we show that the BBB03 locus encodes the B. burg-dorferi telomere resolvase. ResT. The purified protein catalyzes telomere resolution in vitro through a unique reaction: breakage of two phosphodiester bonds in a single DNA duplex (one on each strand) and joining of each end with the opposite DNA strand to form covalently closed hairpin telomeres. Telomere resolution by ResT occurs hairpin telomeres. Telomere resolution by ResT occurs through a two-step transesterification reaction involving the formation of a covalent protein-DNA intermediate at a position three nucleotides from the axis of symmetry in each strand of the substrate.
机译：疏螺旋体属包括莱姆病和复发热的病原体。这些细菌的一个不寻常特征是分段的基因组，主要由许多具有共价闭合的发夹末端或端粒的线性DNA分子组成。在这项研究中，我们表明BBB03基因座编码B. burg-dorferi端粒resolvase。休息。纯化的蛋白质通过独特的反应在体外催化端粒分离：单个DNA双链体中的两个磷酸二酯键断裂（每条链上一个），并且每个末端与相对的DNA链连接形成共价闭合的发夹型端粒。 ResT端粒解析发生发夹式端粒。通过ResT进行的端粒拆分是通过两步酯交换反应发生的，该反应涉及在底物各条链中距对称轴三个核苷酸的位置处形成共价蛋白-DNA中间体。

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