...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular cell >Splicing-Dependent and -Independent Modes of Assembly for Intron-Encoded Box C/D snoRNPs in Mammalian Cells
【24h】

Splicing-Dependent and -Independent Modes of Assembly for Intron-Encoded Box C/D snoRNPs in Mammalian Cells

机译：内含子编码盒C / D snoRNPs在哺乳动物细胞中的拼接依赖和独立的组装模式。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In mammalian cells, all small nucleolar RNAs (snoRNAs) that guide rRNA modification are encoded within the introns of host genes. An optimal position about 70 nts upstream of the 3' splice site of the host intron is critical for efficient expression of box C/D snoRNAs in vivo, suggesting synergy with splicing. Here, we have used a coupled in vitro splicing-snoRNA processing system to demonstrate that assembly of box C/D snoRNP proteins is the step affected by snoRNA location, and that active splicing is essential for snoRNP assembly. Splicing blockage experiments further reveal that snoRNP proteins bind specifically at the spliceosomal C1 complex stage. In contrast, splicing-independent snoRNP assembly can occur in vitro on snoRNAs that possess stable external stems. In vivo analyses confirm that a stable stem can compensate for the unusual position of those few box C/D snoRNAs located far from the 3' splice site of their host intron.
机译：在哺乳动物细胞中，所有指导rRNA修饰的小核仁RNA（snoRNA）均在宿主基因的内含子中编码。宿主内含子3'剪接位点上游约70 nts的最佳位置对于体内框C / D snoRNA的有效表达至关重要，提示与剪接的协同作用。在这里，我们使用了耦合的体外剪接-snoRNA处理系统来证明盒C / D snoRNP蛋白的组装是受snoRNA位置影响的步骤，而主动剪接对于snoRNP组装至关重要。剪接阻断实验进一步揭示了snoRNP蛋白在剪接C1复合体阶段特异性结合。相反，与剪接无关的snoRNP组装可在具有稳定外部茎的snoRNA上在体外发生。体内分析证实，稳定的茎可以弥补远离宿主内含子3'剪接位点的少数几盒C / D snoRNA的异常位置。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号