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首页> 外文期刊>Molecular cell >Rif1 and rif2 inhibit localization of tel1 to DNA ends.
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Rif1 and rif2 inhibit localization of tel1 to DNA ends.

机译：Rif1和rif2抑制tel1在DNA末端的定位。

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摘要

Chromosome ends, known as telomeres, have to be distinguished from DNA double-strand breaks (DSBs) that activate the DNA-damage checkpoint. In budding yeast, the ATM homolog Tel1 associates preferentially with short telomeres and promotes telomere addition. Here, we show that the telomeric proteins Rif1 and Rif2 attenuate Tel1 recruitment to DNA ends through distinct mechanisms. Both Rif1 and Rif2 inhibit the localization of Tel1, but not the Mre11-Rad50-Xrs2 (MRX) complex, to adjacent DNA ends. Rif1 function is weaker at short telomeric repeats compared with Rif2 function and is partly dependent on Rif2. Rif2 competes with Tel1 for binding to the C terminus of Xrs2. Once Tel1 is delocalized, MRX does not associate efficiently with Rap1-covered DNA ends. These results reveal a mechanism by which telomeric DNA sequences mask DNA ends from Tel1 recognition for the regulation of telomere length.
机译：染色体末端（称为端粒）必须与激活DNA损伤检查点的DNA双链断裂（DSB）区分开。在发芽酵母中，ATM同源物Tel1与短端粒优先结合，并促进端粒的添加。在这里，我们显示端粒蛋白Rif1和Rif2通过不同的机制使Tel1募集到DNA末端。 Rif1和Rif2都抑制Tel1定位到相邻DNA末端，但不抑制Mre11-Rad50-Xrs2（MRX）复合物。与Rif2功能相比，在短的端粒重复序列中，Rif1功能较弱，并且部分取决于Rif2。 Rif2与Tel1竞争结合Xrs2的C末端。一旦Tel1脱位，MRX将不会与Rap1覆盖的DNA末端有效结合。这些结果揭示了端粒DNA序列掩盖DNA终止于Tel1识别的机制，以调节端粒长度。

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