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首页> 外文期刊>Molecular cell >Distinct mechanisms for microRNA strand selection by Drosophila Argonautes.
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Distinct mechanisms for microRNA strand selection by Drosophila Argonautes.

机译：果蝇选择microRNA链的不同机制。

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In Drosophila, miRNA strands are predominantly sorted into AGO1 to regulate seed-matched target transcripts, while their partner miRNA* strands are thought to be mostly degraded. Here, we report that Drosophila Argonautes exhibit different strand preferences for miRNA duplexes, and that in particular, many miRNA* species accumulate in the RNAi effector AGO2. AGO2-loaded miRNA* species require canonical RNAi factors for their accumulation, are efficiently 3' modified, and are preferentially active on extensively matched target transcripts. Differential miRNA/miRNA* sorting profiles are correlated with specific central mismatches. In vitro assays revealed an active role for Watson-Crick base-pairing at positions 9 and 10 in promoting strand selection by AGO2, with little reciprocal effect on strand selection by AGO1. We conclude that miRNA strand selection and sorting are actually linked processes that stem from distinct loading preferences of AGO proteins and that independent sorting of duplex strands is a general feature of Drosophila microRNA genes.
机译：在果蝇中，miRNA链主要被分类到AGO1中，以调节种子匹配的靶转录物，而其伴侣miRNA *链则被认为大部分被降解了。在这里，我们报道果蝇的Argonautes对miRNA双链体表现出不同的链偏好，尤其是许多miRNA *物种在RNAi效应子AGO2中积累。载有AGO2的miRNA *物种需要规范的RNAi因子来积累，有效地进行了3'修饰，并在广泛匹配的靶转录本上具有优先活性。差异的miRNA / miRNA *分选谱与特定的中央错配相关。体外试验显示，在第9位和第10位的Watson-Crick碱基配对在促进AGO2促进链选择中发挥了积极作用，而对AGO1进行链选择的影响却很小。我们得出的结论是，miRNA链的选择和排序实际上是由AGO蛋白的不同装载偏好引起的，并且是相互关联的过程，而双链的独立排序是果蝇microRNA基因的普遍特征。

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