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首页> 外文期刊>Molecular cell >RNA-mediated destabilization of the sigma(70) region 4/beta flap interaction facilitates engagement of RNA polymerase by the Q antiterminator
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RNA-mediated destabilization of the sigma(70) region 4/beta flap interaction facilitates engagement of RNA polymerase by the Q antiterminator

机译：RNA介导的sigma（70）区域4 / beta皮瓣相互作用的不稳定促进了Q抗终止剂与RNA聚合酶的结合

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摘要

The bacterial RNA polymerase (RNAP) holoenzyme consists of a catalytic core enzyme (alpha(2)beta'omega) complexed with a sigma factor that is required for promoter-specific transcription initiation. During early elongation, the stability of interactions between sigma(70) (the primary sigma factor in Escherichia colt) and core decreases due to an ordered displacement of segments of sigma(70) from core triggered by growth of the nascent RNA. Here we demonstrate that the nascent RNA-mediated destabilization of an interaction between sigma(70) region 4 and the flap domain of the beta subunit is required for the bacteriophage lambda Q antiterminator protein to contact holoenzyme during early elongation. We demonstrate further that the requirement for nascent RNA in the process by which Q engages RNAP can be bypassed if sigma(70) region 4 is removed. Our findings illustrate how a regulator can exploit the nascent RNA-mediated reconfiguration of the holoenzyme to gain access to the enzyme during early elongation.
机译：细菌RNA聚合酶（RNAP）全酶由与启动子特异的转录起始所需的sigma因子复合的催化核心酶（alpha（2）beta'omega）组成。在早期伸长过程中，由于新生RNA的生长触发了sigma（70）片段从核心开始的有序位移，导致sigma（70）（大肠杆菌中的主要sigma因子）与核心之间相互作用的稳定性降低。在这里，我们证明了新生的RNA介导的σ（70）区域4和β亚基的皮瓣域之间的相互作用的不稳定是噬菌体λQ抗终止剂蛋白在早期延伸过程中接触全酶所必需的。我们进一步证明，如果删除sigma（70）4区，可以绕过Q参与RNAP的过程中对新生RNA的要求。我们的发现说明了调节剂如何在早期延伸过程中利用新生的RNA介导的全酶重组，以获取该酶。

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