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首页> 外文期刊>Molecular cell >A uniform response to mismatches in codon-anticodon complexes ensures ribosomal fidelity
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A uniform response to mismatches in codon-anticodon complexes ensures ribosomal fidelity

机译：对密码子-反密码子复合体错配的统一反应可确保核糖体保真度

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Ribosomes take an active part in aminoacyl-tRNA selection by distinguishing correct and incorrect codon-anticodon pairs. Correct codon-anticodon complexes are recognized by a network of ribosome contacts that are specific for each position of the codon-anticodon duplex and involve A-minor RNA interactions. Here, we show by kinetic analysis that single mismatches at any position of the codon-anticodon complex result in slower forward reactions and a uniformly 1000-fold faster dissociation of the tRNA from the ribosome. This suggests that high-fidelity tRNA selection is achieved by a conformational switch of the decoding site between accepting and rejecting modes, regardless of the thermodynamic stability of the respective codon-anticodon complexes or their docking partners at the decoding site. The forward reactions on mismatched codons were particularly sensitive to the disruption of the A-minor interactions with 16S rRNA and determined the variations in the misreading efficiency of near-cognate codons.
机译：核糖体通过区分正确和不正确的密码子-反密码子对，积极参与氨酰基-tRNA的选择。正确的密码子-反密码子复合物被核糖体接触网络识别，该网络对密码子-反密码子双链体的每个位置都具有特异性，并且涉及A-小分子RNA相互作用。在这里，我们通过动力学分析表明，在密码子-反密码子复合体任何位置的单个错配都会导致正向反应变慢，并且tRNA从核糖体的解离速度均匀提高1000倍。这表明，无论各个密码子-反密码子复合物或其对接伴侣在解码位点的热力学稳定性如何，都可以通过在接受和拒绝模式之间解码位点的构象转换来实现高保真tRNA选择。对错配密码子的正向反应对与16S rRNA的A-小分子相互作用的破坏特别敏感，并确定了近同源密码子误读效率的变化。

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