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首页> 外文期刊>Molecular cell >Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions
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Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions

机译：在不存在启动子区域的Spt6介导的染色质重组的情况下，转录激活子对于转录是必不可少的

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The packaging of the eukaryotic genome into chromatin is likely to have a profound influence on transcription from the underlying genes. We have previously shown that the disassembly of promoter nucleosomes is obligatory for activation of the yeast PHO5 and PHO8 genes. Here, we show that the PHO5 promoter nucleosomes are reassembled concomitant with transcriptional repression and displacement of the TATA binding protein and RNA polymerase II (RNA PoI II). We identify the histone H3-H4 chaperone Spt6 as the factor that mediates nucleosome reassembly onto the PHO5, PHO8, ADH2, ADY2, and SUC2 promoters during transcriptional repression. Furthermore, promoter nucleosome reassembly is essential for transcriptional repression. In the absence of Spt6-mediated nucleosome reassembly, the activators Pho4 and Pho2 are displaced from the PHO5 promoter in repressing conditions, yet transcription is sustained. As such, these studies demonstrate that activators are not required for transcription in the absence of competing chromatin reassembly.
机译：将真核生物基因组包装到染色质中可能对基础基因的转录产生深远影响。先前我们已经表明，启动子核小体的拆卸对于激活酵母PHO5和PHO8基因是必须的。在这里，我们显示PHO5启动子核小体与转录抑制和TATA结合蛋白和RNA聚合酶II（RNA PoI II）的置换同时重组。我们确定组蛋白H3-H4伴侣Spt6为在转录抑制过程中介导PHO5，PHO8，ADH2，ADY2和SUC2启动子上核小体重组的因子。此外，启动子核小体重组对于转录抑制是必不可少的。在没有Spt6介导的核小体重组的情况下，在阻遏条件下，激活剂Pho4和Pho2从PHO5启动子中移出，但转录得以维持。因此，这些研究表明，在不存在竞争性染色质重组的情况下，转录不需要激活剂。

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