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Lactarius ectomycorrhizae on Abies alba: morphological description, molecular characterization, and taxonomic remarks

机译:冷杉(Abies alba)上的乳杆菌外生菌根:形态描述,分子表征和分类学说明

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To date, the ectomycorrhizae formed by silver fir (Abies alba), an ecologically valuable and indigenous tree species in many European mountain forests, have been poorly investigated. We characterized the mycorrhizae formed by three Lactarius species (Lac. subsericatus, Lac. intermedius, Lac. salmon icolor) On silver fir, on the basis of material originating from central Italy. The identification of the fungal symbiont was achieved by means of morphoanatomical observations of mycorrhizae, and by comparison of ITS sequences obtained from mycorrhizae and sporocarps of putative fungal partners. Sequences also were obtained from specimens of the same species but from different geographic origin or from closely related Lactarius species. A maximum likelihood analysis of the data was performed. On the whole, the resultant tree is in good agreement with sporocarp and mycorrhiza morphology. RFLP patterns were calculated from sequence data. A discussion on the main morphoanatomical characters distinguishing the Lactarius ectomycorrhizae reported in this study from those already described belonging to related species, is also included. The accuracy of different methods to identify mycorrhizae formed by closely related Lactarius species on silver fir, are discussed. [References: 52]
机译:迄今为止,对由银冷杉(Abies alba)形成的外生菌根,在许多欧洲山区森林中具有生态价值和本土树种的研究很少。我们基于源自意大利中部的材料,对银冷杉上三个乳杆菌属(乳酸杆菌,中间乳杆菌,中间色鲑鱼)形成的菌根进行了表征。真菌共生体的鉴定是通过对菌根进行形态解剖学观察,并比较从菌根和推定的真菌伴侣的果皮获得的ITS序列来实现的。序列也从相同物种但来自不同地理起源的样品或紧密相关的乳杆菌物种获得。进行了数据的最大似然分析。总体而言,所得到的树与孢子果和菌根形态非常吻合。从序列数据计算RFLP模式。还讨论了区分本研究报道的乳杆菌与已属于相关物种的那些的主要形态解剖学特征。讨论了不同的方法鉴定银杉上紧密相关的乳杆菌物种形成的菌根的准确性。 [参考:52]

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