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首页> 外文期刊>Mycologia >Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture.
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Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture.

机译：淹没液体培养中Pycnoporus sanguineus对漆酶生产的优化。

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摘要

The white-rot fungus Pycnoporus sanguineus produces laccase under a range of C/N ratios in submerged liquid culture. Enzyme production was increased 50 fold in the presence of 20μM xylidine to a maximum of 1368 U/litre in a high carbon low nitrogenmedium. Slight repression of enzyme production was observed in high nitrogen culture medium. Other potential inducers were less effective (Tween 80, wood fibres) and reduced the stimulation observed by xylidine alone when included in the same culture medium. Veratryl alcohol failed to stimulate laccase production. Activity of the enzyme activity in crude culture filtrate was stable at temperatures of 35oC and below with a pH optimum of 3.0. The laccase of P. sanguineus was identified as a c. 65 kDa protein produced as multiple isoforms.
机译：在腐烂的液体培养中，白腐真菌Pycnoporus sanguineus在一定的C / N比范围内产生漆酶。在高碳低氮培养基中，存在20μM二甲苯吡啶的情况下，酶的产量增加了50倍，最高达到1368 U / L。在高氮培养基中观察到酶产生的轻微抑制。当包含在相同的培养基中时，其他潜在的诱导剂效果较差（吐温80，木纤维），并减少了单独使用二甲苯丁所引起的刺激。藜芦醇不能刺激漆酶的产生。粗培养滤液中酶的活性在35oC及以下温度下稳定，最适pH为3.0。 P. sanguineus的漆酶被鉴定为c。 65 kDa蛋白质以多种同工型形式产生。

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