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首页> 外文期刊>Mycologia >In vitro production of hyphal bodies of the mite pathogenic fungus Neozygites floridana.
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In vitro production of hyphal bodies of the mite pathogenic fungus Neozygites floridana.

机译：体外产生螨病原真菌菌丝新菌。

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摘要

The fungus Neozygites floridana is a pathogen commonly associated with the cassava green mite (CGM), Mononychellus tanajoa, and the two-spotted spider mite (TSM), Tetranychus urticae, in South America. Difficulty in culturing N. floridana in vitro has hampered its development as a bioacaricide. A Colombian isolate from TSM was multiplied in TSM and hyphal bodies from infected specimens were inoculated into 18 mycological and tissue culture media. Grace's insect tissue culture medium plus 0.33% lactalbumin hydrolysate and 0.33% yeastolate (GS) afforded vigorous multiplication of hyphal bodies (over 106/ml). The number of hyphal bodies increased with aeration. Final concentration of hyphal bodies was not affected by fetal bovine serum (FBS), but growth was somewhat enhanced. Hyphal bodies produced germ tubes when transferred from GS medium to Grace's medium plus 10% FBS and shaken at 200 rpm for 24 h. The germinated hyphal bodies, when placed on water agar, produced primary conidia from which capilliconidia were produced. These capilliconidia were infective to both TSM and CGM.
机译：真菌Neozygites floridana是一种病原体，通常与南美的木薯绿螨（CGM），塔纳莫尼雪松（Mononychellus tanajoa）和两斑红蜘蛛（TSM）（Tetranychus urticae）有关。弗洛里达猪笼草的体外培养困难阻碍了其作为杀生物杀螨剂的发展。将来自TSM的哥伦比亚分离株在TSM中繁殖，并将感染标本的菌丝体接种到18种真菌学和组织培养基中。格雷斯的昆虫组织培养基加上0.33％的乳清蛋白水解物和0.33％的酵母酸盐（GS）提供了菌丝体的剧烈繁殖（超过106 / ml）。菌丝体的数量随曝气而增加。菌丝体的最终浓度不受胎牛血清（FBS）的影响，但生长有所增强。菌丝体从GS培养基转移到Grace's培养基（含10％FBS）并以200 rpm摇动24 h时产生胚芽管。发芽的菌丝体放在水琼脂上时，会产生初级分生孢子，并从中产生毛状分生孢子。这些毛滴虫病对TSM和CGM均具有感染性。

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