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首页> 外文期刊>Mycologia >Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen. et sp nov., formerly known as dark-septate endophyte Type 1
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Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen. et sp nov., formerly known as dark-septate endophyte Type 1

机译：广泛的根共生冠状青霉（Acephala applanata gen）的分子和表型描述。 et sp nov。，以前称为1型深色分隔内生菌

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摘要

Acephala applanata gen. et sp. nov. is described. A. applanata is a dark-septate endophyte (DSE) of conifer roots and belongs to the Phialocephala fortinii species complex. Several genetic markers, including isozymes, inter-simple-sequence-repeat (ISSR) fingerprints, single-copy restriction fragment length polymorphisms (RFLP) and sequences of the internal transcribed spacers (ITS), let us unambiguously separate isolates of A. applanata from isolates of P fortinii s.l. and other dark-septate endophytes. Alleles at four RFLP loci and two fixed nucleotides in the ITS region were diagnostic for A. applanata. One of the fixed nucleotides resulted in the addition of an Afa I restriction site. PCR amplification with primers prITS4 and the newly developed primer PF-ITS-F (ACT CTG AAT GTT ACT GAT GTC TGA GT) and restriction digestion with Afa I yielded three fragments (203 bp, 117 bp, 56 bp) in A. applanala but only two (260 bp and 117 bp) in P firtinii s.l. Population differentiation (Gs,) between A. applanala and other cryptic species of P fortinii was pronounced, and the index of association (1,) did not deviate significantly from zero, showing that recombination occurs or had occurred in A. applanata. Although isolates of A. applanata never were observed to sporulate, it can be distinguished morphologically from Pfiortinii s.l. by the scarcity of aerial mycelium, significantly slower growth and denser mycelium on cellophane overlaid on water agar. These phenotypic characteristics, combined with diagnostic RFLP alleles and/or PCR-RFLP of the ITS fragment with the fixed Afa I restriction site, unequivocally allow identification of A. applanata.
机译：青头草等。十一月描述。 A. applanata是针叶树根的一种深色分隔的内生植物（DSE），属于披头草（Pialocephala fortinii）种复合体。几个遗传标记，包括同工酶，简单序列重复（ISSR）指纹，单拷贝限制性片段长度多态性（RFLP）和内部转录间隔区（ITS）的序列，使我们明确地将Appliata A. applanata分离自P fortinii sl的分离株和其他深色分隔的内生菌。 ITS区域中四个RFLP位点和两个固定核苷酸处的等位基因可诊断为短吻线虫。固定核苷酸之一导致添加Afa I限制位点。用引物prITS4和新开发的引物PF-ITS-F（ACT CTG AAT GTT ACT GAT GTC TGA GT）进行PCR扩增，并用Afa I限制性酶切产生了A. applanala中的三个片段（203 bp，117 bp，56 bp），但是冷杉小球藻中只有两个（260 bp和117 bp） A. applanala和其他隐性P. fortinii物种之间的种群分化（Gs，）明显，关联指数（1，）没有明显偏离零，表明在A. applanata中发生重组或已经发生重组。尽管从未观察到青霉菌的孢子形成孢子，但是可以从形态学上将其与Pfiortinii s.1区分开。由于缺乏气生菌丝体，覆盖在水琼脂上的玻璃纸上的生长速度明显较慢，菌丝密度较高。这些表型特征，结合具有固定的Afa I限制位点的ITS片段的诊断RFLP等位基因和/或PCR-RFLP，可以明确地鉴定App。applanata。

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