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首页> 外文期刊>Mycologia >Magainin 2 effects on the ultrastructure of five plant pathogens.
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Magainin 2 effects on the ultrastructure of five plant pathogens.

机译：Magainin 2对五种植物病原体的超微结构有影响。

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Magainin 2 from the African clawed frog is a member of a host defence peptide family with broad-spectrum antimicrobial activity related to its effects on membrane integrity. This bioactive peptide was examined for its effects on the ultrastructure of the plant pathogens Thielaviopsis basicola, Rhizoctonia solani, Fusarium oxysporum, Verticillium dahliae and Pythium ultimum. Magainin 2 at 0.05 鎔/鎙itre in potato dextrose broth in microtitre plate wells completely inhibited all hyphal growth observable with a dissecting microscope at 40X, except for P. ultimum. The organisms were subsequently grown on potato dextrose agar and the leading edge of each colony treated with 1 鎔 of magainin 2 in 10 鎙itre. Hyphal plugs from the treated area were takenat 12 and 24 h after treatment for TEM examination. Observations on the ultrastructure of magainin 2-treated hyphae revealed changes in the size and shape of the mitochondria, degradation of the mitochondrial and cytoplasmic matrices, reduction in the number of ribosomes and vacuolization of cytoplasm after 12 h of treatment. The structural degradation of the cells was more severe after 24 h treatment. Magainin 2 at the concn tested had no significant effect on the growth of P. ultimum, perhaps due to the disparate metabolic chemistry of the Oomycetes compared with the other organisms. The low MW and bioactivity of magainin 2 makes it a candidate for additional investigation into its use in bioengineered host resistance.
机译：来自非洲爪蛙的Magainin 2是宿主防御肽家族的成员，该肽具有广谱抗菌活性，与其对膜完整性的影响有关。检查了这种生物活性肽对植物病原菌Thielaviopsis basicola，茄子根瘤菌，尖孢镰刀菌，大黄萎病菌和大腐霉的超微结构的影响。在微量滴定板孔中，马铃薯葡萄糖肉汤中0.05 0.05 /升的Magainin 2完全抑制了用40倍解剖显微镜观察到的所有菌丝的生长，除了终极体育杆菌。该生物随后在马铃薯葡萄糖琼脂上生长，每个菌落的前缘在10微升中用1升magainin 2处理。在治疗后第12和24小时取治疗区的菌丝栓塞进行TEM检查。对用麦加胶蛋白2处理的菌丝的超微结构的观察表明，处理12小时后，线粒体的大小和形状发生变化，线粒体和细胞质基质降解，核糖体数量减少和细胞质空泡化。处理24小时后，细胞的结构降解更为严重。测试中的magainin 2对终极疟原虫的生长没有显着影响，这可能是由于卵菌纲与其他生物相比的代谢化学完全不同。 magainin 2的低分子量和生物活性使其成为进一步研究其在生物工程宿主抗性中的用途的候选人。

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