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DNA diagnostic for Armillaria species in British Columbia: within and between species variation in the IGS-1 and IGS-2 regions

机译:不列颠哥伦比亚省的蜜环菌属物种的DNA诊断:IGS-1和IGS-2地区的物种变异之内和之间

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摘要

Intensive sampling of British Columbia isolates of Armillaria species confirmed the usefulness of a species diagnostic based on restriction site differences in the first ribosomal intergenic spacer region. Greater variation within species and new fragment patterns were observed compared with earlier reports. The only species confusion observed using this marker was between A. gallica and some isolates of A. sinapina. These isolates could be distinguished by restriction site differences in the second intergenic spacer. Use of both sections of the ribosomal intergenic spacer region confirms identifications based on the first intergenic spacer, and provides additional data for species which have the same restriction sites in the first intergenic spacer.
机译:对不列颠哥伦比亚省的蜜环菌属菌种进行大量采样,证实了基于第一个核糖体基因间隔区的限制性位点差异进行物种诊断的有用性。与早先的报告相比,观察到物种内的变化更大,新的碎片模式更明显。使用该标记物观察到的唯一物种混淆是在A. gallica和一些A. sinapina分离株之间。这些分离物可以通过第二基因间隔区中的限制性位点差异来区分。核糖体基因间隔区的两个部分的使用证实了基于第一基因间隔区的鉴定,并提供了在第一基因间隔区中具有相同限制位点的物种的其他数据。

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