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首页> 外文期刊>Molecular reproduction and development >Stage-dependent and alternative splicing of sGnRH messengers in rainbow trout testis during spermatogenesis.
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Stage-dependent and alternative splicing of sGnRH messengers in rainbow trout testis during spermatogenesis.

机译：精子发生过程中虹鳟睾丸中sGnRH信使的阶段依赖性和可变剪接。

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摘要

The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expression of the sGnRH-1 and sGnRH-2 genes encoding salmon GnRH in rainbow trout testis throughout testicular development and spermatogenesis. We demonstrate that both sGnRH mRNA are expressed prior of sexual differentiation. In adult, northern blot analysis indicates that sGnRH-2 transcripts are expressed in the testis at higher levels than sGnRH-1 messengers. Moreover, we observed that the expression of sGnRH-2, and not sGnRH-1, messengers was stage-dependent. sGnRH-2 mRNA expression decreases at the onset and progressively rebounds at the end of spermatogenesis. In addition, we demonstrate that a complex stage-dependent and differential splicing of the sGnRH-2 messengers occurs throughout spermatogenesis. We isolated five transcripts corresponding to sGnRH-2 messengers. Two of them may encode a novel and shortened GnRH-associated peptide containing 18 residues instead of 46. Our data provide new insight in the putative role of GnRH and GAP peptides as autocrine/paracrine factors of spermatogenesis. Copyright 2001 Wiley-Liss, Inc.
机译：促性腺激素释放激素（GnRH）长期以来一直被视为参与生殖周期控制的神经肽。但是，GnRH及其受体在包括卵巢和睾丸在内的各种组织中的存在表明它是自分泌/旁分泌因子。在本研究中，我们报告了虹鳟鱼睾丸发育和精子发生过程中编码鲑鱼GnRH的sGnRH-1和sGnRH-2基因的表达。我们证明这两个sGnRH mRNA表达的性别分化之前。在成人中，Northern印迹分析表明，sGnRH-2转录本在睾丸中的表达水平高于sGnRH-1信使。此外，我们观察到sGnRH-2（而不是sGnRH-1）信使的表达是阶段依赖性的。 sGnRH-2 mRNA表达在生精期开始时降低，并在生精结束时逐渐反弹。另外，我们证明了在整个精子发生过程中，sGnRH-2信使的复杂的阶段依赖性和差异剪接发生了。我们分离了与sGnRH-2信使相对应的五个转录本。其中两个可能编码一个新颖且缩短的GnRH相关肽，其包含18个残基而不是46个残基。我们的数据为GnRH和GAP肽作为精子发生的自分泌/旁分泌因子的推定作用提供了新的见解。版权所有2001 Wiley-Liss，Inc.

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