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首页> 外文期刊>Molecular reproduction and development >Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa.
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Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa.

机译：Tpx-1是大鼠精子的外部致密纤维和顶体的组成部分。

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摘要

Previously we reported the cloning of a member of the cysteine-rich secretory protein family, tpx-1, from a testis expression library using an outer dense fiber (ODF)-specific antiserum. Using immunohistochemical and immunoelectron microscopic techniques and Western blotting of purified sperm tail components, we have determined that tpx-1 exists as 25 and 27 kDa proteins in two components of rat spermatid: the ODFs and the acrosome. Tpx-1 mRNA is first expressed in the late pachytene spermatocytes, but the production of these tpx-1 proteins is translationally delayed for 4-5 days before being incorporated into the developing sperm acrosome, surrounding the elongating and condensing spermatid nucleus. Concurrent with sperm head formation, tpx-1 protein was incorporated into the developing sperm tail, and specifically the ODFs. The tpx-1 protein was seen within structures resembling granulated bodies in the cytoplasmic lobe of elongating spermatids and was incorporated subsequently into the growing tail in a manner consistent with ODF development. In addition, tpx-1 protein was localized at the ultrastructural level of the connecting piece of the neck and longitudinal columns of the fibrous sheath, suggesting common protein components in these cytoskeletal structures. As such, tpx-1 may have functional significance in the processes of sperm head development and tail function.
机译：以前，我们报道了使用外部密集纤维（ODF）特异性抗血清从睾丸表达文库克隆富含半胱氨酸的分泌蛋白家族的成员tpx-1。使用免疫组织化学和免疫电子显微镜技术以及纯化的精子尾部成分的Western印迹，我们已经确定tpx-1以25和27 kDa的蛋白质形式存在于大鼠精子的两个成分中：ODF和顶体。 Tpx-1 mRNA首先在晚期的粗线精原细胞中表达，但是这些tpx-1蛋白的产生被翻译延迟了4-5天，然后被掺入到发育中的精子顶体中，并围绕着精子的伸长和浓缩。与精子头部形成同时，将tpx-1蛋白掺入发育中的精子尾巴中，特别是ODF中。 tpx-1蛋白出现在延长精子细胞质叶状颗粒状结构的内部，随后以与ODF发育一致的方式掺入生长的尾巴中。此外，tpx-1蛋白位于颈连接部和纤维鞘纵柱的超微结构水平，表明这些细胞骨架结构中存在常见的蛋白质成分。这样，tpx-1在精子头部发育和尾巴功能过程中可能具有功能意义。

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