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首页> 外文期刊>Mycologia >Regulation of the cellulase complex production by Saccobolus saccoboloides: induction and repression by carbohydrates
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Regulation of the cellulase complex production by Saccobolus saccoboloides: induction and repression by carbohydrates

机译：葡萄球菌对纤维素酶复合物产生的调节：碳水化合物的诱导和抑制

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摘要

Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular #beta#-glucosidase, #beta#-1,4 endoglucanase and #beta#-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars.
机译：Saccobolus saccoboloides（一种亲真菌）在合成液体培养基中生长。研究了细胞外＃beta＃-葡萄糖苷酶，＃beta＃-1,4内切葡聚糖酶和＃beta＃-1,4外切葡聚糖酶对碳水化合物的诱导和抑制作用。当无法测定内切葡聚糖酶和外切葡聚糖酶时，使用另一种羧甲基纤维素清除方法。结晶纤维素是最好的诱导剂，其次是乳糖。两种诱导剂的混合物均具有意想不到的负面影响。纤维二糖或葡萄糖抑制了含纤维素培养基中纤维素酶的产生。这种抑制对纤维素酶复合物的某些成分具有选择性，使用低于3％w / v的阻遏物浓度未观察到这种抑制。而且，压抑的程度随文化的年龄而变化。这种真菌产生的纤维素酶是可诱导的，并且容易被易代谢的糖类抑制。

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