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首页> 外文期刊>Mycologia >Cephalosporium maydis is a distinct species in the Gaeumannomyces-Harpophora species complex.
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Cephalosporium maydis is a distinct species in the Gaeumannomyces-Harpophora species complex.

机译：头孢霉属是Gaeumannomyces-Harpophora物种复合体中的独特物种。

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摘要

Cephalosporium maydis [Gaeumannomyces graminis var. tritici] is an important plant pathogen whose phylogenetic position relative to other fungi has not been established clearly. We compared strains of C. maydis, strains from several other plant-pathogenic Cephalosporium spp., and several possible relatives within the Gaeumannomyces-Harpophora species complex, to which C. maydis has been suggested to belong based on previous preliminary DNA sequence analyses. DNA sequences of the nuclear genes encoding the rDNA ITS region, beta -tubulin, histone H3, and MAT-2 support the hypothesis that C. maydis is a distinct taxon within the Gaeumannomyces-Harpophora species complex. Based on amplified fragment length polymorphism (AFLP) profiles, C. maydis also is distinct from the other tested species of Cephalosporium, Phialophora sensu lato and members of Gaeumannomyces-Harpophora species complex, which supports its classification as Harpophora maydis [Gaeumannomyces graminis var. tritici]. Oligonucleotide primers for H. maydis were developed that can be used in a PCR diagnostic protocol to rapidly and reliably detect and identify this pathogen. These diagnostic PCR primers will aid the detection of H. maydis in diseased maize because this fungus can be difficult to detect and isolate, and the movement of authentic cultures may be limited by quarantine restrictions.
机译：头孢霉菌[Gaeumannomyces graminis var。小麦是一种重要的植物病原体，其相对其他真菌的系统发生地位尚未明确。我们比较了C. maydis的菌株，其他几种植物致病性头孢菌属的菌株，以及Gaeumannomyces-Harpophora物种复合体中的几种可能的亲缘种，根据先前的初步DNA序列分析，C。maydis属于该物种。编码rDNA ITS区域，β-微管蛋白，组蛋白H3和MAT-2的核基因的DNA序列支持假单胞菌是Gaeumannomyces-Harpophora物种复合体中不同分类群的假设。根据扩增的片段长度多态性（AFLP）谱图，马氏梭状芽胞杆菌还不同于其他测试的头孢菌属，Phialophora sensu lato物种和Gaeumannomyces-Harpophora物种复合体的成员，这支持将其分类为Maypophoris maydis [Gaeumannomyces graminis var。 tritici]。已开发出可用于H.maydis的寡核苷酸引物，该引物可用于PCR诊断方案中，以快速，可靠地检测和鉴定该病原体。这些诊断性PCR引物将有助于检测病态玉米中的H. maydis，因为这种真菌可能难以检测和分离，并且真实培养物的移动可能会受到检疫限制的限制。

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