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首页> 外文期刊>Mycologia >Genetic and morphological characterization of a Fusarium verticillioides conidiation mutant
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Genetic and morphological characterization of a Fusarium verticillioides conidiation mutant

机译：镰刀菌（Fusarium v​​erticillioides）孢子突变体的遗传和形态学表征

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Enteroblastic phialidic conidiation by the corn pathogen Fusarium verticillioides (teleomorph Gibberella moniliformis) produces abundant, mostly single-celled microconidia in distinctive long chains. Because conidia might be critical for establishing in planta associations, we characterized a spontaneous E verticillioides conidiation mutant in which phialides were incapable of enteroblastic conidiogenesis. Instead of producing a conidium, the phialide apex developed a determinate, slightly undulating, germ tube-like outgrowth, in which nuclei rarely were seen. Electron microscopy showed that the apical outgrowth possessed a thick, rough, highly fibrillar outer wall layer that was continuous with the thinner and smoother outer wall layer of the phialide. Both the inner wall layer and plasma membrane also were continuous between the apical outgrowth and phialide. The apical neck region of mutant phialides lacked both a thickened inner wall layer and a wall-building zone, which were critical for conidium initial formation. No indication of septum formation or separation of the apical outgrowth from mutant phialides was observed. These aberrations suggested the apical outgrowth was not a functional conidium of altered morphology. The mutation did not prevent perithecium development and ascosporogenesis. Genetic analyses indicated that a single locus, designated FPH1 (frustrated phialide), was responsible for the mutation. The conidiogenesis mutants were recovered only during certain sexual crosses involving wild-type conidiating parents, and then only in some perithecia, suggesting that mutation of FPH1 might be meiotically induced, perhaps due to mispairing between homologous chromosomes and deletion of the gene from a chromosome.
机译：玉米病原菌Fusarium v​​erticillioides（teleomorph Gibberella moniliformis）产生的成虫卵裂分生孢子在独特的长链中产生大量的单细胞微分生孢子。因为分生孢子可能对于建立植物群落至关重要，所以我们表征了自发的E类小孢子分生突变体，其中phialides无法进行成胚性分生。 phialide尖顶没有产生分生孢子，而是发育出确定的，略微起伏的，胚芽管样的长出，很少见到细胞核。电子显微镜显示，根尖的生长产物具有厚的，粗糙的，高度原纤维状的外壁层，其与phialide的更薄，更光滑的外壁层连续。内壁层和质膜也在顶端产物和phialide之间是连续的。突变的phialides的顶脖子区域既没有增厚的内壁层和墙壁建设区，这是分生孢子最初形成的关键。没有观察到隔膜形成或根尖与突变的phialides分离的迹象。这些畸变表明根尖长出不是形态改变的功能分生孢子。该突变并未阻止皮囊的发育和子囊发生。遗传分析表明，命名为FPH1（沮丧的phialide）的单个基因座负责突变。分生孢子突变体仅在涉及野生型分生双亲的某些性交过程中才恢复，然后仅在某些外周膜中被恢复，这表明FPH1的突变可能是减数分裂诱导的，这可能是由于同源染色体之间的错配和染色体中基因的缺失。

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