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首页> 外文期刊>Mycologia >Phylogenetic analysis of Aspergillus species using DNA sequences from four loci
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Phylogenetic analysis of Aspergillus species using DNA sequences from four loci

机译：利用四个位点的DNA序列对曲霉菌种进行系统发育分析

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摘要

DNA sequences were determined for beta tubulin (BT2), calmodulin (CF), ITS and lsu rDNA (ID) and RNA polymerase II (RPB2) from ca. 460 Aspergillus isolates. RPB2 and rDNA sequences were combined and analyzed to determine relationships in the genus and in the family Trichocomaceae. Eupenicillium species form a statistically supported clade with origins among the Aspergillus clades. A. crystallinus, A. malodoratus and H. paradoxus are members of the Eupenicillium clade. A. zonatus, A. clavatoflvus and W. spinulosa occur in a clade along with Hamigera sp. Other than these exceptional species, Aspergillus species and sections occur on three strongly supported clades that descend from a polytomy. Section Versicolores as a monophyletic group includes only A. versicolor and A. sydowii and is superfluous. The other sections were retained but modified. All four loci were used in genealogical concordance analysis of species boundaries. Fennellia flavipes and F. nivea are not conspecific with their supposed anamorphs A. flavipes and A. nivea. Synonymies were found for some species and more than 20 undescribed taxa were identified in genealogical concordance analysis. Newly discovered taxa will be described elsewhere. Possibly paralogous gene fragments were amplified with the BT2 primers in sections Nidulantes, Usti and Nigri. Use of nonhomologous sequences in genealogical concordance analysis could lead to false conclusions and so BT2 sequences were not used in analysis of those sections.
机译：确定了来自β-微管蛋白（BT2），钙调蛋白（CF），ITS和lsu rDNA（ID）和RNA聚合酶II（RPB2）的DNA序列。 460曲霉菌将RPB2和rDNA序列合并并进行分析，以确定该属与毛果纲科中的关系。 Eupenicillium物种形成了统计支持的进化枝，起源于曲霉进化枝。 A. crystallinus，A。malodoratus和H. paradoxus是Eupenicillium进化枝的成员。 a。zonatus，A。clavatoflvus和W. spinulosa与Hamigera sp。一起出现在进化枝中。除这些例外物种外，曲霉菌种和切片还出现在从多角体切下的三个牢固支撑的进化枝上。 Versicolores节作为单一系统群仅包括A. versicolor和A. sydowii，是多余的。其他部分保留但已修改。所有四个基因座都用于物种边界的族谱一致性分析。 Fennellia flavipes和F. nivea与其假定的无性型A. flavipes和A. nivea没有同种。在某些物种中发现了同义词，并且在族谱一致性分析中识别出20多个未描述的分类单元。新发现的分类单元将在其他地方描述。可能在Nidulantes，Usti和Nigri的区域中使用BT2引物扩增了同源基因片段。在族谱一致性分析中使用非同源序列可能会导致错误的结论，因此在这些部分的分析中未使用BT2序列。

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