• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mycologia >Culturing and direct PCR suggest prevalent host generalism among diverse fungal endophytes of tropical forest grasses
【24h】

Culturing and direct PCR suggest prevalent host generalism among diverse fungal endophytes of tropical forest grasses

机译：培养和直接PCR表明热带森林草的各种真菌内生菌中普遍存在宿主普遍性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Most studies examining endophytic fungi associated with grasses (Poaceae) have focused on agronomically important species in managed ecosystems or on wild grasses in subtropical, temperate and boreal grasslands. However grasses first arose in tropical forests, where they remain a significant and diverse component of understory and forest-edge communities. To provide a broader context for understanding grass-endophyte associations we characterized fungal endophyte communities inhabiting foliage of 11 species of phylogenetically diverse C-3 grasses in the understory of a lowland tropical forest at Barro Colorado Island, Panama. Our sample included members of early-arising subfamilies of Poaceae that are endemic to forests, as well as more recently arising subfamilies that transitioned to open environments. Isolation on culture media and direct PCR and cloning revealed that these grasses harbor species-rich and phylogenetically diverse communities that lack the endophytic Clavicipitaceae known from diverse woodland and pasture grasses in the temperate zone. Both the incidence and diversity of endophytes was consistent among grass species regardless of subfamily, clade affiliation or ancestral habitat use. Genotype and phylogenetic analyses suggest that these endophytic fungi are predominantly host generalists, shared not only among distinctive lineages of Poaceae but also with non-grass plants at the same site.
机译：大多数研究与草（禾本科）有关的内生真菌的研究都集中在管理型生态系统中的农业重要物种或亚热带，温带和北方草原的野草上。但是，草首先出现在热带森林中，在那里它们仍然是林下社区和森林边缘社区的重要组成部分。为了提供更广泛的背景来理解草-内生植物的关联，我们对真菌内生菌群落进行了表征，它们栖息在巴拿马巴罗科罗拉多岛的低地热带森林林下，有11种系统发育多样的C-3草的叶子。我们的样本包括禾本科特有的早熟亚科的成员，它们是森林特有的，以及最近出现的过渡到开放环境的亚科。在培养基上分离并直接进行PCR和克隆显示，这些草种拥有物种丰富且系统发育的群落，缺乏温带地区不同林地和牧草已知的内生草科。不论亚科，进化枝隶属关系或祖先栖息地的使用，草种中内生菌的发生率和多样性都一致。基因型和系统发育分析表明，这些内生真菌主要是宿主通才，不仅在禾本科的独特谱系之间共享，而且与同一地点的非草类植物共享。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号