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Expression of genes of Rhizoctonia solani and the biocontrol Stachybotrys elegans during mycoparasitism of hyphae and sclerotia

机译:菌丝和菌核的支原体寄生性过程中茄根丝线虫和线粒体水生线虫的基因表达

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摘要

Knowledge of mycoparasitism has been focused on how antagonists affect pathogens in relation to mechanisms, metabolites and gene expression. Just as microbial antagonists use a diverse arsenal of mechanisms to dominate interactions with hosts, hosts also have diverse responses to counteract antagonism. In this study differential gene expression of eight mycoparasitism-induced genes and eight host-response genes was monitored during in vivo interactions between the mycoparasite Stachybotrys elegans and hyphae and sclerotia of the host, Rhizoctonia solani over 5 d of interaction. Using real time reverse transcription polymerase chain reaction, comparative analyses demonstrated that hyphal and sclerotial structures triggered different expression patterns. These results indicated that multiple regulatory mechanisms might be involved. The high elevated expression of some genes belonging to the mycoparasite and the host suggest that these genes play an important role during the mycoparasitic process and host defense respectively.
机译:霉菌寄生的知识一直集中在拮抗剂如何影响与机制,代谢产物和基因表达有关的病原体上。就像微生物拮抗剂使用各种机制来控制与宿主的相互作用一样,宿主对对抗性拮抗剂也有多种反应。在这项研究中,在5 d的相互作用过程中,对体内的真菌寄生性水生线虫线虫与宿主的菌丝和菌核的菌丝和菌核之间的体内相互作用进行了监测,检测了八个由真菌副寄生虫诱导的基因和八个宿主响应基因的差异基因表达。使用实时逆转录聚合酶链反应,比较分析表明菌丝和菌核结构触发了不同的表达模式。这些结果表明可能涉及多种调控机制。属于真菌寄生虫和宿主的一些基因的高表达表明,这些基因分别在真菌寄生过程和宿主防御中发挥重要作用。

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