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首页> 外文期刊>Mycologia >Ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with Asparagus officinalis in Andalucia (Spain)
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Ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with Asparagus officinalis in Andalucia (Spain)

机译：Ambispora granatensis，一种新的丛枝菌根真菌，与西班牙安达卢西亚的芦笋相关

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摘要

A new dimorphic fungal species in the arbuscular mycorrhiza-forming Glomeromycota, Ambispora granatensis, was isolated from an agricultural site in the province of Granada (Andalucia, Spain) growing in the rhizosphere of Asparagus officinalis. It was propagated in pot cultures with Trifolium pratense and Sorghum vulgare. The fungus also colonized RI TDNA transformed Daucus carota roots but did not form spores in these root organ cultures. The spores of the acaulosporoid morph are 90-150 mu m diam and hyaline to white to pale yellow. They have three walls and a papillae-like rough irregular surface on the outer surface of the outer wall. The irregular surface might become difficult to detect within a few hours in lactic acid-based mountings but are clearly visible in water. The structural central wall layer of the outer wall is only 0.8-1.5 mu m thick. The glomoid spores are formed singly or in small, loose spore clusters of 2-10 spores. They are hyaline to pale yellow, (25)40-70 mu m diam and have a bilayered spore wall without ornamentation. Nearly full length sequences of the 18S and the ITS regions of the ribosomal gene place the new fungus in a separate clack next to Ambispora fennica and Ambispora gerdemannii. The acaulosporoid spores of the new fungus can be distinguished easily from all other spores in genus Ambispora by the conspicuous thin outer wall.
机译：从生长在芦笋根际的格拉纳达省（西班牙安达卢西亚）的一个农业地点中，分离出了丛枝形成菌根的球孢菌Ambispora granatensis中的一种新的双态真菌。它与Tri叶和高粱一起在盆栽中繁殖。真菌还定殖了RI TDNA转化的胡萝卜胡萝卜根，但在这些根器官培养物中未形成孢子。孢粉状孢子的直径为90-150微米，透明至白色至浅黄色。它们具有三壁和在外壁的外表面上的乳头状粗糙不规则表面。在基于乳酸的固定装置中，不规则的表面可能会在几个小时内变得难以检测，但在水中却清晰可见。外壁的结构中心壁层仅0.8-1.5μm厚。团状孢子单独形成或形成2-10个孢子的小的，松散的孢子簇。它们是透明的至浅黄色，直径为（25）40-70微米，并具有双层孢子壁而无装饰。核糖体基因的18S和ITS区的近乎全长序列将新真菌放置在紧挨着Ambispora fennica和Ambispora gerdemannii的另一个小袋中。新真菌的无孢子孢子可以通过明显的薄外壁轻易地与Ambispora属中的所有其他孢子区分开。

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