• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes
【24h】

Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes

机译：猪卵母细胞生长和成熟过程中胚泡（GV）染色质构型的变化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Changes in germinal vesicle (GV) chromatin configurations during growth and maturation of porcine oocytes were studied using a new method that allows a clearer visualization of both nucleolus and chromatin after Hoechst staining. The GV chromatin of porcine oocytes was classified into five configurations, based on the degree of chromatin condensation, and on nucleolus and nuclear membrane disappearance. While the GV1 to 4 configurations were similar to those reported by previous studies, the GVO configuration was distinct by the diffuse, filamentous pattern of chromatin in the whole nuclear area. Most of the oocytes were at the GVO stage in the < 1 and 1-1.9 mm follicles, but the GVO pattern disappeared completely in the 2-2.9 and 3-6 mm follicles. As follicles grew, the number of oocytes with GV1 configurations increased and reached a maximum in the preovulatory follicles 4 hr post-hCG injection. During maturation in vivo, the number of GV1 oocytes decreased while oocytes undergoing GVBD increased. The percentage of oocytes with GV3 and GV4 configurations was constant during oocyte growth except at the 2-2.9 mm follicle stage, but these configurations disappeared completely after hCG injection. On the contrary, the in vitro maturing oocytes showed a large proportion of GV3 and GV4 configurations. There was no significant difference in distribution of chromatin configurations between the nonatretic and atretic follicles, and between oocytes with more than two layers of cumulus cells and those with less than one layer or no cumulus cells. Overall, our results suggested that (i) the GVO configuration in porcine oocytes corresponded to the "nonsurrounded nucleolus" pattern in mice and other species; (ii) all the oocytes were synchronized at the GV1 stage before GVBD and this pattern might, therefore, represent a nonatretic state; (iii) the GV3 and GV4 configurations might represent stages toward atresia, or transient events prior to GVBD that could be switched toward either ovulation or atresia, depending upon circumstances; (iv) the in vitro systems currently used were not favorable for oocytes to switch toward ovulation (or final maturation); (v) the number of cumulus cells was not correlated with the chromatin configuration of oocytes, indicating that the beneficial effect of cumulus cells on oocyte maturation and development may simply be attributed to their presence during in vitro culture.
机译：使用一种新方法研究了猪卵母细胞生长和成熟过程中胚泡（GV）染色质构型的变化，该方法可以在Hoechst染色后更清晰地观察核仁和染色质。猪卵母细胞的GV染色质基于染色质凝结程度，核仁和核膜消失而分为五种构型。虽然GV1至4的构型与以前的研究报道的相似，但GVO的构型与染色质在整个核区域的弥漫性丝状模式不同。大多数卵母细胞在<1和1-1.9 mm卵泡处处于GVO阶段，但GVO模式在2-2.9和3-6 mm卵泡中完全消失。随着卵泡的生长，hCG注射后4小时，排卵前卵泡中具有GV1构型的卵母细胞数量增加并达到最大值。在体内成熟期间，GV1卵母细胞数量减少，而经历GVBD的卵母细胞增加。除了在2-2.9 mm卵泡期，具有GV3和GV4构型的卵母细胞百分比在卵母细胞生长过程中是恒定的，但是在hCG注射后这些构型完全消失了。相反，体外成熟的卵母细胞显示出很大比例的GV3和GV4构型。在非闭锁卵泡和闭锁卵泡之间，以及卵丘细胞多于两层的卵母细胞与卵丘细胞少于一层或无卵母细胞的卵母细胞之间，染色质构型的分布没有显着差异。总的来说，我们的结果表明：（i）猪卵母细胞中的GVO构型对应于小鼠和其他物种中的“非环绕核仁”模式; （ii）所有卵母细胞在GVBD之前的GV1阶段均已同步，因此该模式可能代表非闭锁状态; （iii）GV3和GV4的配置可能代表闭锁的阶段，或GVBD之前的短暂事件，视情况而定，可以转换为排卵或闭锁; （iv）目前使用的体外系统不利于卵母细胞转向排卵（或最终成熟）; （v）卵丘细胞的数目与卵母细胞的染色质构型不相关，表明卵丘细胞对卵母细胞成熟和发育的有益作用可能仅归因于它们在体外培养过程中的存在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号