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首页> 外文期刊>Molecular reproduction and development >Regulation of Prostaglandin Synthesis in Ovaries of Sexually-Mature Zebrafish (Danio rerio)
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Regulation of Prostaglandin Synthesis in Ovaries of Sexually-Mature Zebrafish (Danio rerio)

机译：性斑马鱼卵巢中前列腺素合成的调控

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This study investigates the regulation of prostaglandin (PG) synthesis in the ovaries of sexually-mature zebrafish (Danio rerio). We examined the ovarian expression of genes within the arachidonic acid (AA) pathway, and the ovarian levels of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-P), 17 beta-estradiol (E-2), and PGF(2 alpha) in spawning and nonspawning fish during the ovulatory cycle. Real-time RT-PCR analysis revealed that the expression levels of cytosolic phospholipase A(2) (cpla2) and cyclooxygenases (COX)-2 (ptgs2) in ovarian fragments and in isolated full-grown follicles of spawning fish were highest at 6:00 when ovulation was expected to occur. In nonspawning fish, cpla2 expression levels declined over time while ptgs2 expression displayed the same temporal pattern as in spawning fish. Elevated levels of 17 alpha,20 beta-P in the spawning fish occurred at 3:30, but there were no changes in the nonspawning fish. In other studies conducted to investigate the hormonal regulation of AA pathway genes, fish exposed via the water for 24 or 96 hr to 17 alpha,20 beta-P or E-2 exhibited reduced ovarian expression levels of COX-1 (ptgs1) and PG E synthase-2 (ptgsl), and E-2 reduced the expression of cpla2. Injection of human chorionic gonadotropin (hCG) (100 IU) led to increased expression levels of cpla2 and ptgs2 at 2 and 18 hr post-treatment, but consistently reduced ptgsl and ptgsl expression. In these fish, ovarian levels of 17 alpha,20 beta-P were elevated at all time points and PGF(2 alpha) levels in the hCG-treated group were significantly higher than the control fish at 18 hr. Collectively, these in vivo results suggest that gonadotropins and steroids are involved in the regulation of the AA pathway in ovarian follicles of zebrafish. Mol. Reprod. Dev. 76: 1064-1075, 2009.
机译：这项研究调查了性成熟斑马鱼（Danio rerio）卵巢中前列腺素（PG）合成的调控。我们检查了花生四烯酸（AA）途径内基因的卵巢表达，以及卵巢中的17 alpha，20 beta-dihydroxy-4-pregnen-3-one（17 alpha，20 beta-P），17 beta-雌二醇的水平（E-2）和排卵周期中产卵鱼和非产卵鱼中的PGF（2 alpha）。实时RT-PCR分析显示，卵巢碎片和产卵的独立完整卵泡中胞质磷脂酶A（2）（cpla2）和环氧合酶（COX）-2（ptgs2）的表达水平最高为6：预期排卵时为00。在非产卵鱼类中，cpla2表达水平随时间下降，而ptgs2表达显示出与产卵鱼类相同的时间模式。产卵鱼中17α，20 beta-P水平升高于3:30发生，但非产卵鱼没有变化。在其他研究中，研究了AA通路基因的激素调控，鱼类在水中暴露于17 alpha，20 beta-P或E-2暴露于水中24或96小时后，卵巢中的COX-1（ptgs1）和PG表达水平降低。 E合酶2（ptgs1）和E-2降低了cpla2的表达。注射人绒毛膜促性腺激素（hCG）（100 IU）导致在治疗后2小时和18小时cpla2和ptgs2的表达水平增加，但始终降低ptgs1和ptgs1的表达。在这些鱼中，在所有时间点卵巢水平都升高了17 alpha，20 beta-P，在hCG治疗组中，在18小时时PGF（2 alpha）水平明显高于对照鱼。这些体内结果共同表明，促性腺激素和类固醇参与了斑马鱼卵巢卵泡中AA途径的调节。大声笑责备。开发人员76：1064-1075，2009。

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