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首页> 外文期刊>Molecular reproduction and development >Temperature effects on sex determination and ontogenetic gene expression of the aromatases cyp19a and cyp19b, and the estrogen receptors esr1 and esr2 in atlantic halibut (Hippoglossus hippoglossus)
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Temperature effects on sex determination and ontogenetic gene expression of the aromatases cyp19a and cyp19b, and the estrogen receptors esr1 and esr2 in atlantic halibut (Hippoglossus hippoglossus)

机译：温度对大西洋大比目鱼（Hippoglossus hippoglossus）的芳香酶cyp19a和cyp19b以及雌激素受体esr1和esr2的性别决定和个体发育基因表达的影响

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摘要

The aromatase (CYP19) and estrogen receptor (ESR) play important roles in the molecular mechanism of sex determination and differentiation of lower vertebrates. Several studies have proven these mechanisms to be temperature sensitive, which can influence the direction of phenotypic gender development. A temperature study was conducted to examine the effect of temperature on the sex differentiation in farmed Atlantic halibut. Sexually undifferentiated larvae were exposed to 7 degrees C, 10 degrees C, or 13 degrees C during gonadal differentiation. Temperature effects on the transcription rate of the aromatase genes cyp19a (ovary type) and cyp19b (brain type) and the ESR genes esr1 and esr2 were examined by quantitative real-time PCR. With increasing temperatures, both cyp19a mRNA levels and the female incidence showed a decreasing trend, thus strongly indicating a relation between the expression of cyp19a and morphological ovary differentiation. In contrast to cyp19a, the levels of cyp19b, esr1, and esr2 mRNA strongly increased in all temperature groups throughout the study period, and did not show obvious temperature-related expression patterns. The present data provide evidence that posthatching temperature exposure significantly affects the expression of cyp19a mRNA during the developmental period and that high temperature possibly influences genetic sex determination in Atlantic halibut. Though, the female incidence never exceeded 50%, suggesting that only the homogametic (XX) female is thermolabile. So whereas temperature treatment is not likely suitable for direct feminization in halibut, the possibility for high-temperature production of XX neomales for broodstock to obtain all-female offspring by crossing with XX females is suggested. Mol. Reprod. Dev. 73: 1481-1490, 2006. (c) 2006 Wiley-Liss, Inc.
机译：芳香酶（CYP19）和雌激素受体（ESR）在性别决定和低等脊椎动物分化的分子机制中起着重要作用。几项研究证明了这些机制对温度敏感，这可能影响表型性别发展的方向。进行了一项温度研究，以研究温度对养殖大西洋大比目鱼性别分化的影响。在性腺分化过程中，未分化的性幼虫暴露于7摄氏度，10摄氏度或13摄氏度。通过实时定量PCR检测温度对芳香酶基因cyp19a（卵巢型）和cyp19b（脑型）以及ESR基因esr1和esr2转录速率的影响。随着温度的升高，cyp19a mRNA水平和女性发病率均呈下降趋势，从而强烈表明cyp19a的表达与卵巢形态分化之间的关系。与cyp19a相反，在整个研究期间，在所有温度组中cyp19b，esr1和esr2 mRNA的水平均显着增加，并且未显示明显的温度相关表达模式。本数据提供了证据，表明孵化后的温度暴露在发育期间会显着影响cyp19a mRNA的表达，而高温可能会影响大西洋大比目鱼的遗传性别决定。但是，女性发病率从未超过50％，这表明只有同配（XX）女性是不耐热的。因此，尽管温度处理不太可能适合在大比目鱼中直接进行女性化，但建议通过与XX雌性杂交，高温生产XX新雄性亲虾以获得全雌性后代的可能性。大声笑责备。开发人员73：1481-1490，2006。（c）2006 Wiley-Liss，Inc.

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