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首页> 外文期刊>Mycologia >Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium
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Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium

机译：Emb Embsia和Nimbya物种的分子表征及其与链格孢属，Ulocladium和Stemphylium的关系

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摘要

DNA sequences from rDNA and proteincoding regions were determined for six Embellisia and two Nimbya spp. and were compared to those from Alternaria, Ulocladium and Stemphylium spp. Sequences determined included rDNA from the nuclear internal transcribed-spacer region (ITS1/5.8S/ ITS2) and the mitochondrial small-subunit (mt SSU) and a portion of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene. Phylogenetic analyses were performed on each dataset separately and then combined for total evidence analysis using methods of maximum parsimony and maximum likelihood. Results revealed that Embellisia and Nimbya clustered within a large monophyletic Alternaria-Nimbya-Embellisia-Ulocladium clade with Stemphylium as the sister taxon. Members of the infectoria species-group were the most basal group in this large polygeneric clade. Embellisia and Nimbya were sister taxa of the remaining Alternaria and Udocladium spp. and were related more closely to Alternaria than was Stemphylium. Four Embellisia spp. formed a monophyletic clade. However, E. allii clustered with the two Nimbya spp. and E. indefessa clustered with Alternaria and Ulocladium spp., revealing that Embellisia, as currently circumscribed, is polyphyletic. Potential revisions of taxonomy for all genera are discussed.
机译：确定来自rDNA和蛋白质编码区的DNA序列，用于六个Embellisia和两个Nimbya spp。并将其与链格孢属，Ulocladium和Stemphylium spp的那些进行比较。确定的序列包括来自核内转录间隔区（ITS1 / 5.8S / ITS2）和线粒体小亚基（mt SSU）的rDNA，以及部分甘油醛-3-磷酸脱氢酶（gpd）基因。对每个数据集分别进行了系统发育分析，然后使用最大简约和最大似然方法进行总证据分析。结果显示，Embellisia和Nimbya聚集在一个大型单生的Alternaria-Nimbya-Embellisia-Ulocladium进化枝中，其中以Stemphylium为姊妹类群。在这个大型多基因进化枝中，感染菌种组的成员是最基础的群体。 Embellisia和Nimbya是剩下的Alternaria和Udocladium spp的姐妹类群。并且与茎线虫的关系比茎线虫更紧密。四个Embllsia spp。形成了单一的进化枝。但是，大肠埃希菌与两个宁比亚属物种聚在一起。 E. indefessa和Alternaria和Ulocladium spp。聚类，表明目前所限的Embellisia是多系的。讨论了对所有属的分类学的潜在修订。

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