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Phytophthora species in forest streams in Oregon and Alaska

机译:俄勒冈和阿拉斯加森林溪流中的疫霉菌种

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Eighteen Phytophthora species and one species of Halophytophthora were identified in 113 forest streams in Alaska, western Oregon and southwestern Oregon that were sampled by baiting or filtration of stream water with isolation on selective media. Species were identified by morphology and DNA characterization with single strand conformational polymorphism, COX spacer sequence and ITS sequence. ITS Clade 6 species were most abundant overall, but only four species, P. gonapodyides (37% of all isolates), P. taxon Salixsoil, P. taxon Oaksoil and P. pseudosyringae, were found in all three regions. The species assemblages were similar in the two Oregon regions, but P. taxon Pgchlamydo was absent in Alaska and one new species present in Alaska was absent in Oregon streams. The number of Phytophthora propagules in Oregon streams varied by season and in SW Oregon, where sampling continued year round, P. taxon Salixsoil, P. nemorosa and P. siskiyouensis were recovered only in some seasons.
机译:在阿拉斯加,俄勒冈州西部和俄勒冈州西南部的113个森林溪流中鉴定出18种疫霉属和一种嗜盐藻属,通过诱集或过滤流水中的水进行采样,并在选择性培养基上进行分离。通过形态和DNA表征,利用单链构象多态性,COX间隔区序列和ITS序列鉴定物种。 ITS进化枝6种总体上最为丰富,但在这三个区域中仅发现了4个种,即角ap对虾(占分离株的37%),紫杉,柳杉,紫杉和假丁香。两个俄勒冈州的物种组成相似,但是阿拉斯加不存在P.taxon Pgchlamydo,而俄勒冈州河流中不存在阿拉斯加存在的一种新物种。俄勒冈州溪流中疫霉菌繁殖体的数量随季节和俄勒冈州西南部的不同而有所变化,该州全年不断采样,仅在某些季节才恢复了紫杉,柳杉,线虫和siskiyouensis。

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