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首页> 外文期刊>Mycologia >Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto
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Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto

机译：ITS rDNA序列的种内和基因组内变异揭示了严寒仔藻的分类学问题

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摘要

Fourteen new species in the Latin American Glade (LAC) of the Ceratocystis fimbriata complex recently were distinguished from C. fimbriata sensu stricto largely based on variation in ITS rDNA sequences. Among the 116 isolates representing the LAC, there were 41 ITS haplotypes. Maximum, parsimony (MP) analysis of ITS sequences produced poorly resolved trees. In contrast, analyses of matingtype genes (MAT1-1-2 and MAT1-2-1) resolved a single MP tree with branches of high bootstrap and posterior probability support. Four isolates showed intragenomic variation in ITS sequences. Cloning and sequencing of PCR products from a single haploid strain identified two or more ITS sequences differing at up to 16 base positions and representing two described species. Isolates from introduced populations that appeared to be clonal based on microsatellite markers varied at up to 14 bp in ITS sequence. Strains of seven Brazilian ITS haplotypes and an isolate from Ipomoea batatas (on which the species name C. fimbriata was based) were fully interfertile in sexual crosses. These analyses support three phylogenetic species that differ in pathogenicity: C. platani, C. cacaofunesta and C. colombiana. Five ITS species (C. manginecans, C. mangicola, C. mangivora, C. acaciivora, C. eucalypticola) appear to be ITS haplotypes that have been moved from or within Brazil on nursery stock. The taxonomic status of other species delineated primarily by ITS sequences (C. diversiconidia, C. papillata, C. neglecta, C. ecuadoriana, C. fimbriatomima, C. curvata) needs further study, but they are considered doubtful species.
机译：最近，主要根据ITS rDNA序列的差异，严格区分了Ceratocystis fimbriata复合体的拉丁美洲Glade（LAC）中的14个新物种。在代表LAC的116个分离株中，有41个ITS单倍型。 ITS序列的最大简约（MP）分析产生了分辨率较差的树。相比之下，对交配型基因（MAT1-1-2和MAT1-2-1）的分析解析出了具有高自举和后验概率支持的单个MP树。四个分离株显示出ITS序列中的基因组内变异。来自单个单倍体菌株的PCR产物的克隆和测序确定了两个或多个ITS序列，这些序列在最多16个碱基位置处不同，代表两个描述的物种。来自引入群体的分离物似乎是基于微卫星标记而克隆的，其ITS序列最高可达14 bp。有七个巴西ITS单倍型的菌株和一个来自番薯（Ipomoea batatas）的分离株（种名是C. fimbriata就是基于该菌株）在性交中完全受干扰。这些分析支持三种致病性不同的系统发育物种：白金梭菌，C.cacaofunesta和C. colombiana。 ITS单倍型似乎有5种ITS种类（C. manginecans，C。mangicola，C。mangivora，C。aciciivora，C。eucalypticola），它们是从苗圃中移出或移出巴西的ITS单倍型。主要由ITS序列描述的其他物种（C. diversiconidia，C。papillata，C。neglecta，C。ecuadoriana，C。fimbriatomima，C。curvata）的分类状况需要进一步研究，但它们被认为是可疑物种。

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