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A new ophiostomatoid genus from Protea infructescences

机译:普罗梯亚茶的新的类镜蛇类属

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摘要

In recent years, 2 unusual ophiostomatoid fungi, Ceratocystiopsis proteae and Ophiostoma capense, have been described from infructescences of Protea spp. They are unique in having Knoxdaviesia anamorphs and differ from each other in ascospore morphology. Both species are sensitive to cycloheximide, typical of Ceratocystis sensu stricto. RFLP analyses on the rRNA operon regions of the type species of Ceratocystis, Ophiostoma and Ceratocystiopsis, as well as C. proteae and O. capense, were conductedusing the restriction enzymes Eco RI, Hae II, Pst I, Sal I, Sma I and Xba I. By constructing restriction maps, phylogenetic relationships between these species were determined. From the data, it was evident that C. proteae and O. capense are phylogenetically unrelated to both Ceratocystis and Ophiostoma, but showed a closer affinity to Ceratocystis than to Ophiostoma. On the basis of the RFLP analyses, cycloheximide sensitivity, cell saccharides and anamorph morphology, a new genus, Gondwanamyces, is established to accommodate these 2 unusual fungi.
机译:近年来,从Protea spp的花序发育中描述了2种不寻常的蛇眼类真菌,Ceratocystiopsis蛋白酶和Ophiostoma capense。它们在具有诺克斯氏畸形的过程中是独特的,并且在子囊孢子形态上彼此不同。两种物种均对严格的塞拉样囊虫(Ceratocystis sensu stricto)特有的环己酰亚胺敏感。使用限制性内切酶Eco RI,Hae II,Pst I,Sal I,Sma I和Xba进行了对Ceratocystis,Ophiostoma和Ceratocystiopsis类型物种的rRNA操纵子区域以及C.proteae和O.capense的RFLP分析。 I.通过构建限制性图谱,确定了这些物种之间的系统发育关系。从数据来看,很明显,蛋白酶C.capense和C. capense与Ceratocystis和Ophiostoma在系统发育上都不相关,但是与Cophatocystis的亲和力比与Ophiostoma更强。在RFLP分析的基础上,建立了环己酰亚胺的敏感性,细胞糖和无性形态,建立了一种新的菌群,以容纳这两种不寻常的真菌。

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