...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >Carbohydrate components of lipovitellin of the sand crab Emerita asiatica.
【24h】

Carbohydrate components of lipovitellin of the sand crab Emerita asiatica.

机译：沙蟹Emerita asiatica的脂蛋白的碳水化合物成分。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipovitellin II (Lv II), the major yolk protein of the anomuran crab Emerita asiatica, was purified using heparin-sepharose affinity column chromatography. The purified Lv II was a glycoprotein as it was stainable with periodic acid-Schiff's reagent. Quantitative analysis of sugars showed the presence of fucose, mannose, galactosamine, N-linked oligosaccharides, as well as O-linked oligosaccharides containing N-acetyl hexosamine as the terminal residue. The amount of N-linked oligosaccharides is higher than that of the O-linked oligosaccharides. Biogel P-4 column chromatographic separation of the radiolabeled oligosaccharides of Lv II showed the presence of five different O-linked oligosaccharides and four different N-linked oligosaccharide species. HPTLC separation of the neoglycolipids prepared from the O-linked oligosaccharides also showed the presence of five different O-linked oligosaccharide species. N-linked oligosaccharides contain significant quantities of mannose. Unisil column chromatographic purification in conjunction with HPTLC separation revealed three neutral glycolipid species such as monoglycosylceramide, diglycosylceramide, and triglycosylceramide in the Lv II. The functional significance of these carbohydrate components of the major yolk protein during embryogenesis of the sand crab is discussed.
机译：使用肝素-琼脂糖亲和柱色谱法纯化无头蟹蟹Emerita asiatica的主要蛋黄蛋白Lipovitellin II（Lv II）。纯化的Lv II是一种糖蛋白，因为它可以用高碘酸-席夫氏试剂染色。糖的定量分析表明存在岩藻糖，甘露糖，半乳糖胺，N-连接的寡糖以及含有N-乙酰基六胺作为末端残基的O-连接的寡糖。 N-连接寡糖的量高于O-连接寡糖的量。 Lv II放射性标记的寡糖的Biogel P-4柱色谱分离表明，存在5种不同的O-连接的寡糖和4种不同的N-连接的寡糖。由O-连接寡糖制备的新糖脂的HPTLC分离也显示存在五种不同的O-连接寡糖。 N-连接的寡糖含有大量的甘露糖。 Unisil柱色谱纯化与HPTLC分离相结合，揭示了Lv II中的三种中性糖脂类，例如单糖基神经酰胺，二糖基神经酰胺和三糖基神经酰胺。讨论了卵黄主要蛋白的这些碳水化合物组分在沙蟹胚胎发生过程中的功能意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号