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Rhizophydium brooksianum sp nov., a multipored chytrid from soil

机译:根瘤菌(Rhizophydium brooksianum sp nov。),一种来自土壤的多孔菌丝

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摘要

Rhizophydium136 was isolated from pollen bait placed in a water culture containing garden soil from Penobscot County, Maine. It is an important isolate because its entire mitochondrial genome has been sequenced and it is the representative member of the Chytridiales in a fungal phylogeny based on mitochondrial protein sequences. Also, this isolate Is included in an 18S rDNA, chytrid phylogeny. On nutrient agar, many inflated rhizoidal axes extend from the base of the zoosporangium, zoosporangia mature in 3 d and zoospores discharge through numerous, lenticular, discharge pores. Smooth-walled resting spores form in crowded cultures. Zoospores are a variation of the Rhizophydium subtype. This chytrid differs from R. sphaerolheca sensu Barr and because it cannot be placed in a described species it herein is described as Rhizophydium brooksianum sp. nov. Many of the differences between Rhizophydium brooksianum and other multipored Rhizophydium isolates were observed only in pure culture. Attributing a spherical, multipored Rhizophydium to a species that was described without developmental information front pure culture is untenable. Epitypes or neotypes for inadequately characterized species need to be selected, and cultures made available.
机译:根瘤菌136是从花粉诱饵中分离出来的,该花粉诱饵被放置在来自缅因州佩诺布斯科特县的含有花园土壤的水培中。它是重要的分离物,因为已对其整个线粒体基因组进行了测序,并且它是基于线粒体蛋白序列的真菌系统发育中the虫的代表性成员。另外,此分离物也包含在18S rDNA chytrid系统发育中。在营养琼脂上,许多膨大的根状轴从游动孢子虫的底部延伸,游动孢子虫在3 d内成熟,游动孢子通过无数的双凸状喷孔排出。壁厚的静息孢子在拥挤的文化中形成。游动孢子是根瘤菌亚型的变体。该chytrid不同于R.sphaerolheca sensu Barr,并且由于不能放置在所述物种中,因此在本文中被称为Rhizophydium brooksianum sp。十一月仅在纯培养物中才观察到了根瘤菌根瘤菌和其他多孔根瘤菌分离物之间的许多差异。将球形,多孔根瘤菌归因于没有发展信息的物种,而不进行纯培养。需要选择特征不足的物种的表型或新型,并提供培养物。

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