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首页> 外文期刊>Mycologia >Penicillium species endophytic in coffee plants and ochratoxin A production
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Penicillium species endophytic in coffee plants and ochratoxin A production

机译：咖啡植物内生青霉菌种和and曲霉毒素A的产生

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摘要

Tissues from Coffea arabica, C. congensis, C. dewevrei and C. liberica collected in Colombia, Hawaii and at a local plant nursery in Maryland were sampled for the presence of fungal endophytes. Surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. DNA was extracted from a set of isolates visually recognized as Penicillium, and the internal transcribed spacer region and partial LSU-rDNA was amplified and sequenced. Comparison of DNA sequences with GenBank and unpublished sequences revealed the presence of 11 known Penicillium species: P. brevicompactum, P. brocae, P. cecidicola, P. citrinum, P. coffeae, P. crustosum, P. janthinellum, P. olsonii, P. oxalicum, P. sclerotiorum and P. steckii as well as two possibly undescribed species near P. diversum and P. roseopurpureum. Ochratoxin A was produced by only four isolates, one isolate each of P. brevicompactum, P. crustosum, P. olsonii and P. oxalicum. The role these endophytes play in the biology of the coffee plant remains enigmatic.
机译：从阿拉伯咖啡，C。congensis，C。dewevrei和C. liberica的组织中收集了在哥伦比亚，夏威夷和马里兰州的一个本地苗圃中采集的真菌内生菌的组织。将表面消毒过的组织（包括根，叶，茎和各种浆果部分）铺在酵母麦芽琼脂上。从视觉上被识别为青霉的一组分离物中提取DNA，并对内部转录的间隔区和部分LSU-rDNA进行扩增和测序。将DNA序列与GenBank和未公开的序列进行比较后发现，存在11种已知的青霉菌种：短小青霉菌，短小青霉菌，cecidicola，青柠菌，咖啡菌，十字花果菌，janthinellum，P。olsonii， P. oxalicum，P。sclerotiorum和P. steckii以及P. diversum和P. roseopurpureum附近的两个可能未描述的物种。 ch曲霉毒素A仅由四个分离物产生，短小毕赤酵母，克鲁氏假单胞菌，olsonii和草酸假单胞菌各分离一个。这些内生菌在咖啡植物的生物学中发挥的作用仍然是谜。

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