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首页> 外文期刊>Mycologia >GDP-mannose transporter paralogues play distinct roles in polarized growth of Aspergillus nidulans.
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GDP-mannose transporter paralogues play distinct roles in polarized growth of Aspergillus nidulans.

机译：GDP-甘露糖转运蛋白类似物在构巢曲霉的极化生长中起不同的作用。

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摘要

GDP-mannose transporters (GMT) carry GDP-mannose nucleotide sugars from the cytosol across the Golgi apparatus membrane for use as substrates in protein glycosylation in plants, animals and fungi. Genomes of some fungal species, such as the yeast Saccharomyces cerevisiae, contain only one gene encoding a GMT, while others, including Aspergillus nidulans, contain two (gmtA and gmtB). We previously showed that cell wall integrity and normal hyphal morphogenesis in A. nidulans depend upon the function of GmtA and that GmtA localizes to a Golgi-like compartment. Cells bearing the calI11 mutation in gmtA also have reduced cell surface mannosylation. Here we show that GmtB colocalizes with GmtA, suggesting that the role of GmtB is similar to that of GmtA, although the respective transcript levels differ during spore germination and early development. Transcript levels of gmtB are high in ungerminated spores and remain so throughout the first 16 h of germination. In contrast, transcript levels of gmrtA are negligible in ungerminated spores but increase to levels comparable to those of gmtB during germination. These observations suggest that although GmtA and GmtB reside within the same subcellular compartments, they nevertheless perform distinct functions at different stages of development.
机译：GDP-甘露糖转运蛋白（GMT）从细胞质中通过高尔基体膜携带GDP-甘露糖核苷酸糖，用作植物，动物和真菌中蛋白质糖基化的底物。一些真菌物种的基因组，例如酵母酿酒酵母，仅包含一个编码GMT的基因，而其他基因（包括构巢曲霉）则包含两个基因（gmtA和gmtB）。我们以前表明，构巢曲霉的细胞壁完整性和正常菌丝形态取决于GmtA的功能，并且GmtA定位于高尔基体样区室。在gmtA中带有calI11突变的细胞也具有减少的细胞表面甘露糖基化作用。在这里，我们显示GmtB与GmtA共定位，这表明GmtB的作用类似于GmtA，尽管在孢子萌发和早期发育过程中各自的转录水平不同。未发芽的孢子中gmtB的转录水平很高，并且在发芽的前16小时一直保持如此。相反，在未发芽的孢子中，gmrtA的转录水平可忽略不计，但在发芽过程中，其水平增加到与gmtB相当。这些观察结果表明，尽管GmtA和GmtB驻留在相同的亚细胞区室中，但它们在不同的发育阶段执行不同的功能。

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