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Revision of Trichoderma sect. Longibrachiatum including related teleomorphs based on analysis of ribosomal DNA internal transcribed spacer sequences.

机译:修订木霉属。基于对核糖体DNA内部转录的间隔区序列的分析,长臂纲动物包括相关的远形体。

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Variation within the internal transcribed spacer (ITS-1 and ITS-2) regions of ribosomal DNA of 103 strains was studied to examine relationships within Trichoderma sect. Longibrachiatum and related teleomorphs, Hypocrea schweinitzii and H. jecorina.The 4 species, T. longibrachiatum, T. pseudokoningii, T. parceramosum and T. citrinoviride, that were originally assigned by morphological criteria to this section were recognized by sequence analysis. Trichoderma reesei, which was previously consideredto be synonymous with T. longibrachiatum and T. saturnisporum which was placed originally in sect. Saturnisporum because of its warted conidia, are assigned to the section. ITS sequences of T. ghanense, originally assigned to sect. Saturnisporum, could not be distinguished from the sequence of T. parceramosum, indicating that the 2 may be synonymous. Trichoderma "todica", an unpublished name based on the antiviral strain NRRL 3091, is T. parceramosum. Interspecific variability within sect. Longibrachiatum was low (2.2-4.8% in ITS-1, 0-10.2% in ITS-2) when compared with species of other sections. Phylogenetic analysis showed strong support for the monophyly of sect. Longibrachiatum. The Hypocrea teleomorphs with anamorphs that could be assigned to sect.Longibrachiatum fall into at least 6 in part geographically defined groups on the basis of ITS sequences. The anamorphs of three of these groups can be assigned to T. longibrachiatum, T. citrinoviride and T. pseudokoningii, respectively. Variability among these teleomorph groups is in the same order of magnitude as among the 6 anamorph species of sect. Longibrachiatum. Hypocrea jecorina and T. reesei showed 100% sequence homology. Classification derived from ITS sequences is discussed in comparison with results from other molecular methods.
机译:研究了103株核糖体DNA的内部转录间隔区(ITS-1和ITS-2)区域内的变异,以检查木霉属内的关系。经序列分析将最初通过形态学标准分配给该部分的4个种,即长假丝酵母,假单胞菌,假丝酵母​​和柠檬酸丁酸杆菌,归类于本节中的Longibrachiatum和相关的变型体Hypocrea schweinitzii和H.jecorina。里氏木霉(Trichoderma reesei),以前被认为是最初被放置在该教派中的longibrachiatum T.和saturnisporum的同义词。土生孢因其弯曲的分生孢子而被分配到该部分。 T. ghanense的ITS序列,最初分配给该种。不能将Saturnisporum与T. parceramosum的序列区分开,表明2可能是同义词。木霉“ todica”是基于抗病毒株NRRL 3091的未公开名称,它是T. parceramosum。种内种间变异性。与其他部分的种类相比,Longibrachiatum的含量较低(ITS-1为2.2-4.8%,ITS-2为0-10.2%)。系统发育分析表明对该科的单性有很强的支持。 Longibrachiatum。可以被归类为长臂金龟的变形体的次剑突变种根据ITS序列至少分为6个部分地理定义的组。这些组中的三个的变形可以分别分配给长支原木,柠檬酸衣原体和假单胞菌。这些变语组之间的变异与该教派的6个无性变种中的变异幅度相同。 Longibrachiatum。红褐肉座菌和里氏木霉表现出100%的序列同源性。与其他分子方法的结果相比较,讨论了从ITS序列得出的分类。

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