• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mycologia >Restriction analysis of internal transcribed spacers and the small subunit gene of ribosomal DNA among four Gaeumannomyces species.
【24h】

Restriction analysis of internal transcribed spacers and the small subunit gene of ribosomal DNA among four Gaeumannomyces species.

机译：内部转录间隔区和四个Gaeumannomyces种核糖体DNA小亚基基因的限制性分析。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 18S rDNA and the region spanning the 2 internal transcribed spacers (ITS), which includes the 5.8S rDNA, from 36 isolates of 4 species of Gaeumannomyces (G. graminis, G. incrustans, G. cylindrosporous and G. leptosporous) were amplified using the polymerase chain reaction. The amplified products were subjected to restriction enzyme digestion. The restriction patterns in the ITS regions separated both the species of Gaeumannomyces and the 3 varieties of G. graminis. Specific variation in restriction patterns of the ITS regions was observed within the isolates of G. graminis var. graminis. Physical maps of the restriction sites in the ITS region were constructed. Variation among isolates of G. graminis var. tritici and G. graminis var. avenae was minimal. The 3' end and 5' end of the 18S rDNA are conserved among varieties of G. graminis and isolates of G. incrustans. The middle region of the nuclear 18S rDNA amplified with NS3/NS6 primers showed 4 different lengths and based on these data the species and varieties of Gaeumannomyces were arranged into 5 different groups. Group 1 had a length of 1.6 Kb and included isolates of G. graminis var. tritici, G. graminis var. avenae and G. graminis var. graminis isolate WF9463; group 2 (1.4 Kb) included G. graminis var. graminis isolates WF9238 and WF9469; group 3 (1.2 Kb) included isolates of G. incrustans and G. graminis var. graminis isolates WF9236, WF9237 and WF9454; group 4 (1.0 Kb) included G. graminis var. graminis isolates WF9014, WF921, WF9452, WF9455, WF9457, WF9462, WF9470 and WF9471 and finally group 5 included G. cylindrosporous and G. leptosporous with a 1.1 Kb length.
机译：扩增了来自4个Gaeumannomyces（G. graminis，G。incrustans，G。cylindrosporous和G. leptosporous）的36种分离物的18S rDNA和跨越2个内部转录间隔区（ITS）的区域，其中包括5.8S rDNA。使用聚合酶链反应。对扩增产物进行限制性内切酶消化。 ITS区域中的限制模式将Gaeumannomyces种和G. graminis的3个品种分开。在G. graminis var。的分离物中观察到ITS区域的限制性模式的特定变化。 Graminis。绘制了ITS区限制酶切位点的物理图谱。 G. graminis var分离株之间的变异。 Tritici和G. graminis var。燕麦是最小的。 18S rDNA的3'端和5'端在G. graminis和G. incrustans的分离株中是保守的。用NS3 / NS6引物扩增的18S rDNA核的中间区域显示4个不同的长度，并根据这些数据将Gaeumannomyces的种类和品种分为5个不同的组。第一组的长度为1.6 Kb，包括分离的G. graminis var。特里蒂奇（G. graminis var。） avenae和G. graminis var。 graminis分离WF9463;第2组（1.4 Kb）包括G. graminis var。 graminis分离WF9238和WF9469;第3组（1.2 Kb）包括分离的G. incrustans和G. graminis var。 graminis分离株WF9236，WF9237和WF9454;第4组（1.0 Kb）包括G. graminis var。 graminis分离株WF9014，WF921，WF9452，WF9455，WF9457，WF9462，WF9470和WF9471，最后的第5组包括长度为1.1 Kb的圆柱孢菌和瘦孢菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号