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首页> 外文期刊>Mycologia >RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato
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RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato

机译：RAPD-PCR分析马铃薯和番茄中链格孢菌和链格孢菌的遗传变异

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摘要

RAPD-PCR analysis was used to investigate genetic variation among 35 isolates of A. solani, the causal agent of early blight on solanaceous crops, and 30 isolates of A. alternata, an opportunistic pathogen. A symmetric dissimilarity matrix, based onEuclidean metric distances, was used to calculate interpopulation genetic distances (phist) between the fungal species, geographic origin, and host plant. Of the total genetic variation observed, 34% (phist=0.340, P<0.0001) resided between the 2 fungalspecies. A very highly significant interpopulation genetic distance (phist=0.2275, P<0.0001) was observed between isolates of A. solani collected from potatoes and tomatoes, suggesting the possibility of pathogenic specialization. There was a significantdifference between isolates of A. solani (phist=0.1569, P<0.017) and A. alternata (phist=0.1096, P<0.02) from foreign and domestic sources, but no difference between isolates from Pennsylvania and other states. One primer, P248, generated polymorphismsthat could be used to distinguish between the 2 fungal species.
机译：RAPD-PCR分析用于调查35种茄科农作物早疫病的病原体sol。A. solani和机会病原体A. alternata的30种细菌之间的遗传变异。基于欧氏距离的对称不相似矩阵用于计算真菌物种，地理起源和寄主植物之间的种群间遗传距离（phist）。在观察到的全部遗传变异中，有34％（phist = 0.340，P <0.0001）居住在两个真菌物种之间。从马铃薯和番茄中收集到的土壤链霉菌分离株之间观察到非常高的种群间遗传距离（phist = 0.2275，P <0.0001），这表明有可能进行病原专业化。国外和国内来源的sol。A. solani（phist = 0.1569，P <0.017）和A. alternata（phist = 0.1096，P <0.02）的分离株之间存在显着差异，但宾夕法尼亚州和其他州的分离株之间没有差异。一种引物P248产生了可用于区分2种真菌物种的多态性。

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