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首页> 外文期刊>Mycologia >SEPARATION OF TILLETIA INDICA CHROMOSOMES USING CHEF GEL ELECTROPHORESIS
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SEPARATION OF TILLETIA INDICA CHROMOSOMES USING CHEF GEL ELECTROPHORESIS

机译：应用CHEF GEL电泳分离印度小花T虫染色体

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Electrophoretic karyotypes were determined for single teliospore isolates and monosporidial lines of Tilletia indica, causal agent of Karnal bunt disease of wheat. Good separation of chromosomes of T. indica was achieved using 0.7% chromosomal grade agarose and a 3-15 minute ramped switching interval for 168 hours at 60 volts. At least 11 chromosomes were observed in T. indica, ranging in size from ca 1 to over 3.3 megabases. Many isolates contained unique karyotypes. Substantial variation in banding intensity was observed within many isolates, indicating the possible presence of multiple chromosomes within single bands. Electrophoretic karyotypes of single teliospore isolates from infected wheat seeds were compared with those of monosporidial isolates derived therefrom. Some differences were observed in karyotypes of teliospore and monosporidial lines, indicating that alterations in karyotype may occur through meiosis in T. indica. Southern blot hybridization revealed that the large subunit ribosomal DNA gene is located on the largest chromosome in T. indica. [References: 13]
机译：确定了Tilletia indica（小麦Karnal bunt病的病原体）的单个孢子分离株和单孢子线的电泳核型。使用0.7％染色体级琼脂糖和60V电压下3-15分钟的倾斜切换间隔168小时，可实现T稻染色体的良好分离。在印度T中观察到至少11条染色体，大小从ca 1到超过3.3兆碱基。许多分离株含有独特的核型。在许多分离株中观察到条带强度的显着变化，表明在单个条带中可能存在多个染色体。将来自被感染小麦种子的单个孢子分离株的电泳核型与衍生自其的单孢子分离株的电泳核型进行比较。在端粒和单孢子线的核型中观察到一些差异，表明核型的改变可能是通过印度T的减数分裂发生的。 Southern印迹杂交表明，大的亚基核糖体DNA基因位于印度T虫的最大染色体上。 [参考：13]

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