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Viability and maturation of Aphanomyces cochlioides oospores

机译：Aphanomyces cochlioides卵孢子的生存能力和成熟度

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Plasmolysis, tetrazolium bromide staining and microscopic appearance were tested for their usefulness in determining viability of oospores of Aphanomyces cochhoides. For comparison, three lethal treatments were employed to contrast the reaction of dead oospores and untreated, presumably viable oospores. Few oospores stained with tetrazolium bromide, even though plasmolysis and microscopic appearance indicated that 85% were viable. Cytoplasm of viable oospores was densely organized and uniformly granular (DOUG), whereas cytoplasm of oospores exposed to lethal treatments was loosely organized and non-uniformly granular (LONG). Dose-response bioassay experiments were conducted with untreated oospores of varying inoculum densities or with mixtures of untreated DOUG and heat-treated LONG oospores in varying proportions. The number of DOUG oospores was correlated (R-2 = 0.62, P < 0.001) with severity of damping-off of sugar beet seedlings caused by A. cochhoides. Thus, the granular appearance of cytoplasm offered a fast, easy and reliable indicator of viability of A. cochlioides oospores. Tests with newly formed oospores/oogonia showed that >80% harvested at 3-4 d after inoculation of hypocoryls stained with tetrazolium, but by 8-9 d < 10% stained, apparently because of declining permeability of the spore wall to tetrazolium as oospores matured. [References: 17]
机译：测试了血浆溶解，溴化四氮唑染色和显微外观在确定Aphanomyces cochhoides卵孢子存活力方面的有用性。为了进行比较，采用了三种致死性处理来对比死孢子和未处理的大概是活的孢子的反应。很少有卵孢子被溴化四唑染色，尽管溶质分析和显微镜观察表明85％是可行的。存活的卵孢子的细胞质被密集地组织并且均匀地呈颗粒状（DOUG），而暴露于致死性处理的卵孢子的细胞质被疏松地组织成不均匀的颗粒状（LONG）。用不同接种量的未处理卵孢子或未处理的DOUG和热处理的长卵孢子的混合物按不同比例进行剂量反应生物测定实验。 DOUG卵孢子的数量与A.cochhoides引起的甜菜幼苗的衰减严重程度相关（R-2 = 0.62，P <0.001）。因此，细胞质的颗粒状外观提供了一种快速，简便和可靠的指示圆孢曲霉卵菌活力的指标。对新形成的卵孢子/卵原进行的测试表明，接种四唑鎓的次胚轴后3-4 d收获量> 80％，但染色后的8-9 d <10％，这显然是因为孢子壁对作为卵孢子的四唑鎓的渗透性下降成熟了[参考：17]

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