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首页> 外文期刊>Mycologia >Phialocephala urceolata, sp nov., from a commercial, water-soluble heparin solution
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Phialocephala urceolata, sp nov., from a commercial, water-soluble heparin solution

机译：Phialocephala urceolata，sp nov。，来自商业的水溶性肝素溶液

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摘要

Phialocephala urceolata sp. nov. was isolated from a black film that had developed on a water-soluble proprietary heparin solution (pH 2.5). Morphological and enzymatic characters, along with phylogenetic analyses of rDNA sequence data, indicated that the conidial fungus is closely related to species of Phialocephala known primarily as endophytes in the roots of vascular plants (e.g. Acephala applanata, P. fortinii and P. sphaeroides) or as associates of persistent plant organs such as the stems and needles of woody plants (e.g. P. compacta, P. dimorphospora and P. scopiformis). Phialocephala urceolata is distinctive in having urn-shaped phialides that are sparsely distributed along the conidiophore axis, a slow growth rate in culture and in exhibiting a unique combination of reactions on enzymatic test media (i.e. it acidifies casamino acids medium and is gelatinase negative). Partial sequence data from the small subunit (SSU) rDNA indicated that P. urceolata is among the Helotiales and close to the type species of Phialocephala. Sequence data from the internal transcribed spacer (ITS) region places P. urceolata closest to P. sphaeroides. The source of this contaminant is unknown but its taxonomic relationship with other root endophytic species and its ability to produce polyphenol oxidases suggest that the natural habitat of this species is possibly woody plant tissues or soil enriched with lignocellulose.
机译：毛头虫十一月从在水溶性专有肝素溶液（pH 2.5）上显影的黑色薄膜中分离出油。形态学和酶学特征，以及对rDNA序列数据的系统发育分析表明，分生孢子真菌与鳞翅目真菌的种类密切相关，而鳞翅目真菌主要是维管植物根部的内生菌（例如无头蕨，fortinii和phasphaeroides）或作为持久性植物器官的伴生，例如木本植物的茎和针（例如P. compacta，P。dimorphospora和P. scopiformis）。毛头虫的独特之处在于其骨形的phialides沿子生孢子轴稀疏分布，培养速度缓慢，并且在酶促测试培养基上表现出独特的反应组合（即酸化酪蛋白氨基酸培养基，明胶酶阴性）。来自小亚基（SSU）rDNA的部分序列数据表明，P。urceolata在Helotiales中，并且接近于Phialocephala的类型物种。来自内部转录间隔区（ITS）区域的序列数据将P. urceolata放置在最接近球形球菌的地方。该污染物的来源尚不清楚，但其与其他根内生物种的分类学关系及其产生多酚氧化酶的能力表明，该物种的自然栖息地可能是木本植物组织或富含木质纤维素的土壤。

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