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Entrophospora nevadensis, a new arbuscular mycorrhizal fungus from Sierra Nevada National Park (southeastern Spain)

机译:内华达山脉国家公园(西班牙东南部)的一种新型丛枝菌根真菌Entrophospora nevadensis

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摘要

A new fungal species in the arbuscular mycorrhiza-forming Glomeromycetes, Entrophospora nevadensis, was isolated from soil near the roots of several endemic and endangered plant species (e.g. Plantago nivalis and Alchemilla fontqueri) growing in Sierra Nevada National Park (Granada, Andalucia, Spain). The fungus was propagated in trap cultures on Plantago nivalis and Sorbus hybrida and in pure cultures on Trifolium pratense and Sorghum vulgare. Spores are yellow brown to brown, 90-115 mu m diam and form singly in soil, in the neck of adherent sporiferous saccules that form either terminally or intercalary on mycelial hyphae. Spores have two three-layered walls and conspicuous, 6-12 mu m long, spiny, thorn-like projections on the outer wall consisting of hyaline to subhyaline, evanescent tips and yellow brown to brown, persistent bases. In aging spores these projections are usually shorter (1-2.8 mu m) and dome-shaped or rounded, sometimes with a central pit on top where the evanescent tip has sloughed off. Molecular analysis with partial sequences of the 18S ribosomal gene places the fungus within the Diversisporales. The new fungus was found in soil near plants with different living strategies but growing in high altitude soils with acidic pH, high soil moisture and organic carbon content, and close to streams.
机译:内华达山脉国家公园(格拉纳达,安达卢西亚,西班牙)生长的几种特有和濒危植物物种(如车前草和雪莲)的根部附近的土壤中分离出了形成丛枝菌根的glomeromycetes中的一种新真菌物种Entrophospora nevadensis。 。该真菌在车前草和山梨中的诱集培养物中繁殖,在Tri叶和高粱上也以纯培养物繁殖。孢子为黄棕色至棕色,直径90-115微米,仅在土壤中形成,形成在附着的孢子囊的颈部,孢子在菌丝菌丝的末端或or生。孢子有两层三层壁,外壁上有明显的,6-12微米长的,多刺的,刺状的突起,由透明的至亚透明质的,淡淡的尖端和黄棕色至棕色的持久基部组成。在老化的孢子中,这些突起通常较短(1-2.8微米),呈圆顶状或圆形,有时在顶部有一个中央凹坑,where逝尖端已脱落。用18S核糖体基因部分序列进行的分子分析将真菌置于Diversisporales中。在具有不同生存策略的植物附近的土壤中发现了这种新真菌,但这种真菌在酸性pH值,高土壤水分和有机碳含量并靠近溪流的高海拔土壤中生长。

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