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首页> 外文期刊>Mycologia >Development of a specific polymerase chain reaction assay for the detection of Basidiobolus
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Development of a specific polymerase chain reaction assay for the detection of Basidiobolus

机译：用于检测巴西杆菌的特异性聚合酶链反应分析方法的开发

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摘要

The etiology of chronic diarrhea is complex in humans and animals. It is always necessary to evaluate a list of differential diagnosis, including bacteria, protozoa and fungi. Basidiobolornycosis is a fungal disease reported sporadically worldwide, mainly caused by B. rartarum, a frequent organism found in soil or in the intestine and skin of lizards and frogs. It is an opportunistic pathogen that causes infections characterized by granulomatous lesions in the subcutaneous tissues as well as in the intestinal wall in humans and animals. In this work we have developed a PCR technique to differentiate Basidiobolus from other causes of intestinal disease in dogs and humans. To test the specificity of the PCR assay we included closely related organisms, common intestinal microbiota and pathogenic organisms, such as Aspergillus, Candida, Ctyplosporidium, Escherichia, Giardia, Mucor, Proteus, Rhizopus and Salmonella. Pythium insidiosum, which cause clinically similar disease in dogs but require a different treatment. Only Basidiobolus was positive to the PCR assay.
机译：在人类和动物中，慢性腹泻的病因很复杂。始终需要评估鉴别诊断清单，包括细菌，原生动物和真菌。巴氏杆菌属真菌病是世界范围内偶发报道的一种真菌病，主要由牛毛芽孢杆菌引起，牛毛芽孢杆菌是一种常见的生物，存在于土壤，蜥蜴和青蛙的肠和皮肤中。它是一种机会病原体，可在人和动物的皮下组织以及肠壁中引起肉芽肿性病变。在这项工作中，我们已经开发了一种PCR技术，可将巴西双歧杆菌与狗和人的其他肠道疾病原因区分开。为了测试PCR检测的特异性，我们包括密切相关的生物，常见的肠道菌群和病原性生物，例如曲霉菌，念珠菌，梭菌，大肠埃希菌，贾第虫，Mucor，变形杆菌，根霉和沙门氏菌。致死性腐霉（Pythium insidiosum），在犬中引起临床上相似的疾病，但需要不同的治疗方法。仅巴西杆菌对PCR测定呈阳性。

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